Two target regions of allelic loss on chromosome 9 in urinary-bladder cancer.
Two target regions of allelic loss on chromosome 9 in urinary-bladder cancer.
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DOI：
10.1111/j.1349-7006.1999.tb00841.x
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发表时间：
1999-09
期刊：
影响因子：
--
通讯作者：
Emi M
中科院分区：
文献类型：
--
作者：
Ohgaki K;Minobe K;Kurose K;Iida A;Habuchi T;Ogawa O;Kubota Y;Akimoto M;Emi M
9号染色体上的等位基因丢失在多种人类肿瘤中是常见的;此外，一些遗传性癌症综合征的两个易感基因座，即，家族性恶性黑色素瘤和Gorlin综合征已在该染色体上被鉴定。为了确定膀胱癌中假定的肿瘤抑制基因的位置，我们检测了85例膀胱癌9号染色体上18个微卫星位点的等位基因丢失。还寻求9号染色体杂合性丢失与几个临床病理参数之间的相关性。在54个肿瘤（64%）中观察到等位基因丢失，缺失图谱确定了两个靶区域;一个在9 p21上的间隔处，两侧是D9 S736和D9 S165，另一个在9 q31 - 34上的间隔处，两侧是D9 S58和D9 S61。PTCH基因位于后一个区间，未检测到细微突变。在低级别和非侵袭性肿瘤以及更晚期表型的肿瘤中经常观察到9号染色体上的等位基因丢失。位于9号染色体上两个常见缺失区域之一的肿瘤抑制基因的失活可能会影响膀胱肿瘤发展早期的致癌机制。
Allelic losses on chromosome 9 are common in a wide variety of human tumors; moreover, two predisposing loci for some inherited cancer syndromes, i.e., familial malignant melanoma and Gorlin syndrome, have been identified on this chromosome. To define the location of putative tumor suppressor genes involved in cancer of the urinary bladder, 85 bladder cancers were examined for allelic loss at 18 microsatellite loci on chromosome 9. Correlations were also sought between loss of heterozygosity on chromosome 9 and several clinicopathological parameters. Allelic loss was observed in 54 of the tumors (64%) and deletion mapping identified two target regions; one at an interval on 9p21 flanked by D9S736 and D9S165, and the other at an interval on 9q31‐34 flanked by D9S58 and D9S61. No subtle mutation was detected in the PTCH gene which lies in the latter interval. Allelic loss on chromosome 9 was observed frequently in low grade and non‐invasive tumors as well as in tumors of more advanced phenotype. Inactivation of tumor suppressor genes lying in either of two regions of common deletion identified on chromosome 9 might affect carcinogenic mechanisms at an early stage of tumor development in the urinary bladder.
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影响因子：
8.8
作者：
Brewster, S. F.;Gingell, J. C.;Browne, S.;Brown, K. W.
通讯作者：
Brown, K. W.
影响因子：
4.4
作者：
Habuchi, T;Luscombe, N;Knowles, MA
通讯作者：
Knowles, MA
影响因子：
8.8
作者：
Björkqvist AM;Tammilehto L;Anttila S;Mattson K;Knuutila S
通讯作者：
Knuutila S
影响因子：
8
作者：
McGarvey, TW;Maruta, Y;Malkowicz, SB
通讯作者：
Malkowicz, SB
影响因子：
30.8
作者：
BUETOW, KH;WEBER, JL;MURRAY, JC
通讯作者：
MURRAY, JC