Oral Bromelain Attenuates Inflammation in an Ovalbumin-induced Murine Model of Asthma.
Oral Bromelain Attenuates Inflammation in an Ovalbumin-induced Murine Model of Asthma.
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DOI：
10.1093/ecam/nel110
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发表时间：
2008-03
期刊：
影响因子：
--
通讯作者：
Thrall RS
中科院分区：
文献类型：
--
作者：
Secor ER;Carson WF;Singh A;Pensa M;Guernsey LA;Schramm CM;Thrall RS
菠萝蛋白酶是一种广泛使用的菠萝提取物，具有半胱氨酸蛋白酶活性，已被证明在多种免疫系统模型中具有免疫调节作用。本研究的目的是确定口服菠萝蛋白酶对卵清蛋白 (OVA) 诱导的急性过敏性气道疾病 (AAD) 小鼠模型的影响。为了建立 AAD，雌性 C57BL/6J 小鼠用腹膜内 (i.p.) OVA/明矾致敏，然后用 OVA 气雾剂攻击 3 天。从OVA气溶胶攻击前1天开始，连续四天每天两次用（磷酸盐缓冲盐水）PBS或200mg/kg菠萝蛋白酶对小鼠进行强饲。比较治疗组之间的气道反应性和乙酰甲胆碱敏感性、支气管肺泡灌洗 (BAL) 细胞差异、Th2 细胞因子 IL-5 和 IL-13 以及肺组织学。与 PBS 对照相比，口服菠萝蛋白酶治疗 AAD 小鼠显示出治疗效果，表现为乙酰甲胆碱敏感性降低 (P ≤ 0.01)、BAL 嗜酸性粒细胞 (P ≤ 0.02) 和 IL-13 浓度 (P ≤ 0.04) 减少。此外，与对照组相比，口服菠萝蛋白酶显着减少了 AAD 小鼠 BAL CD19+ B 细胞 (P ≤ 0.0001) 和 CD8+ T 细胞 (P ≤ 0.0001)。这些结果表明，菠萝蛋白酶的口服治疗在这种哮喘小鼠模型中具有有益的治疗效果，并且菠萝蛋白酶也可能对人类疾病有效。
Bromelain, a widely used pineapple extract with cysteine protease activity, has been shown to have immunomodulatory effects in a variety of immune system models. The purpose of the present study was to determine the effects of orally administered bromelain in an ovalbumin (OVA)-induced murine model of acute allergic airway disease (AAD). To establish AAD, female C57BL/6J mice were sensitized with intraperitoneal (i.p.) OVA/alum and then challenged with OVA aerosols for 3 days. Mice were gavaged with either (phosphate buffered saline)PBS or 200 mg/kg bromelain in PBS, twice daily for four consecutive days, beginning 1 day prior to OVA aerosol challenge. Airway reactivity and methacholine sensitivity, bronchoalveolar lavage (BAL) cellular differential, Th2 cytokines IL-5 and IL-13, and lung histology were compared between treatment groups. Oral bromelain-treatment of AAD mice demonstrated therapeutic efficacy as evidenced by decreased methacholine sensitivity (P ≤ 0.01), reduction in BAL eosinophils (P ≤ 0.02) and IL-13 concentrations (P ≤ 0.04) as compared with PBS controls. In addition, oral bromelain significantly reduced BAL CD19+ B cells (P ≤ 0.0001) and CD8+ T cells (P ≤ 0.0001) in AAD mice when compared with controls. These results suggest that oral treatment with bromelain had a beneficial therapeutic effect in this murine model of asthma and bromelain may also be effective in human conditions.
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DOI：
10.1152/ajpgi.1997.273.1.g139
发表时间：
1997-07-01
影响因子：
4.5
作者：
Castell, JV;Friedrich, G;Poppe, GE
通讯作者：
Poppe, GE
影响因子：
10
作者：
Hijazi, N;Abalkhail, B;Seaton, A
通讯作者：
Seaton, A
影响因子：
12.4
作者：
Bratke, K.;Haupt, F.;Virchow, J. C., Jr.
通讯作者：
Virchow, J. C., Jr.
DOI：
10.1080/02652030500199439
发表时间：
2005-12-01
影响因子：
2.9
作者：
Durgnat, JM;Heuser, J;Perrin, C
通讯作者：
Perrin, C
影响因子：
46.9
作者：
Gollin, MA
通讯作者：
Gollin, MA