Metabolic Phenotyping and Strain Characterisation of Pseudomonas aeruginosa Isolates from Cystic Fibrosis Patients Using Rapid Evaporative Ionisation Mass Spectrometry.
Metabolic Phenotyping and Strain Characterisation of Pseudomonas aeruginosa Isolates from Cystic Fibrosis Patients Using Rapid Evaporative Ionisation Mass Spectrometry.
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DOI：
10.1038/s41598-018-28665-7
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发表时间：
2018-07-19
影响因子：
4.6
通讯作者：
Takáts Z
中科院分区：
文献类型：
--
作者：
Bardin EE;Cameron SJS;Perdones-Montero A;Hardiman K;Bolt F;Alton EWFW;Bush A;Davies JC;Takáts Z
快速蒸发电离质谱（REIMS）是一种用于生物材料实时分析的新技术。它的工作原理是通过样品传导电流，使其快速加热和蒸发，并将含有蒸汽的分析物引导至质谱仪。它用于表征从囊性纤维化 (CF) 患者分离的 45 种铜绿假单胞菌 (P. aeruginosa) 的代谢组，并与 80 种非 CF 铜绿假单胞菌进行比较。磷脂信号强度最高；检测到 17 种鼠李糖脂和 18 种群体感应分子，表明 REIMS 具有研究毒力相关代谢物的潜力。从呼吸道样本中获得的铜绿假单胞菌分离株表现出较高的多样性，这归因于大多数呼吸道感染的慢性性质。 REIMS 的分析灵敏度允许检测代谢组，该代谢组可用于在重复培养后对单个铜绿假单胞菌分离株进行分类，准确度为 81%，与多位点序列分型的平均一致性为 83%。这项研究巩固了 REIMS 作为临床应用工具的能力，例如重要 CF 病原体铜绿假单胞菌的代谢表型分析，并强调了代谢指纹图谱在亚种水平上进行精细尺度表征的潜力。
Rapid evaporative ionisation mass spectrometry (REIMS) is a novel technique for the real-time analysis of biological material. It works by conducting an electrical current through a sample, causing it to rapidly heat and evaporate, with the analyte containing vapour channelled to a mass spectrometer. It was used to characterise the metabolome of 45 Pseudomonas aeruginosa (P. aeruginosa) isolates from cystic fibrosis (CF) patients and compared to 80 non-CF P. aeruginosa. Phospholipids gave the highest signal intensity; 17 rhamnolipids and 18 quorum sensing molecules were detected, demonstrating that REIMS has potential for the study of virulence-related metabolites. P. aeruginosa isolates obtained from respiratory samples showed a higher diversity, which was attributed to the chronic nature of most respiratory infections. The analytical sensitivity of REIMS allowed the detection of a metabolome that could be used to classify individual P. aeruginosa isolates after repeated culturing with 81% accuracy, and an average 83% concordance with multilocus sequence typing. This study underpins the capacities of REIMS as a tool with clinical applications, such as metabolic phenotyping of the important CF pathogen P. aeruginosa, and highlights the potential of metabolic fingerprinting for fine scale characterisation at a sub-species level.
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影响因子：
11.8
作者：
Livermore, DM
通讯作者：
Livermore, DM
影响因子：
3.6
作者：
Boles, BR;Thoendel, M;Singh, PK
通讯作者：
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作者：
Calfee, MW;Shelton, JG;Pesci, EC
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作者：
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