Oviduct acid mucus glycoproteins in the estrous rabbit: ultrastructure and histochemistry.

Oviduct acid mucus glycoproteins in the estrous rabbit: ultrastructure and histochemistry.
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发情兔输卵管酸性粘液糖蛋白:超微结构和组织化学。

DOI:
10.1095/biolreprod26.1.155
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发表时间:
1982
影响因子:
3.6
通讯作者:
Bajpai,VK
Bajpai,VK
中科院分区:
生物学2区
文献类型:
--
作者:
Jansen,RP;Bajpai,VK

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上皮糖蛋白在生殖的许多方面可能是重要的。兔输卵管产生粘液糖蛋白。组织化学和输卵管运输过程中兔卵周围的粘液被胶凝化都表明了这一点。我们在这里报告,高酸性粘液糖蛋白(显然是海岸卵子的类型)的生产仅限于峡部,并在较小程度上,壶腹峡部交界处的粘膜隐窝,壶腹不参与。使用灌注固定的方法,包括聚阳离子阿尔新蓝结合戊二醛沉淀和稳定的糖蛋白,我们已经证明,这种粘液,至少在发情期的兔子,占据峡腔,但不是壶腹腔。组织化学显示,它是峡部和壶腹峡部交界处的电子透明分泌颗粒,而不是壶腹的致密颗粒,表现出高酸性粘液糖蛋白的染色特征。在峡部运输过程中,峡部粘液与精子和受精卵的相互作用可能存在重要的机会。
Epithelial glycoproteins are likely to be important in many aspects of reproduction. The rabbit oviduct produces mucus glycoproteins. This is indicated both by histochemistry and by gelation of a mucus coat around the rabbit ovum during its tubal transport. We report here that the production of highly acid mucus glycoprotein (apparently of the type that coast the ovum) is confined to the isthmus and, to a lesser extent, the mucosal crypts of the ampullary-isthmic junction; the ampulla is not involved. Using a method of perfusion-fixation that includes the polycation alcian blue in conjunction with glutaraldehyde to precipitate and stabilize glycoproteins, we have demonstrated that this mucus, at least in rabbits in estrus, occupies the isthmic lumen but not the ampullary lumen. Histochemistry shows that it is the electron-lucent secretory granules of the isthmus and ampullary-isthmic junction, but not the denser granules of the ampulla, that exhibit staining characteristics of highly-acid mucus glycoproteins. Important opportunities are likely to exist for interaction of this isthmic mucus with spermatozoa and with fertilized ova during their isthmic transport.
糖蛋白结构的当前概念
DOI: --
发表时间: 1978
期刊:
影响因子: --
作者:
E. Walborg
通讯作者: E. Walborg
DOI: 10.1002/jez.1401350305
发表时间: 1957
期刊: The Journal of experimental zoology
影响因子: --
作者:
Gilbert S. Greenwald
通讯作者: Gilbert S. Greenwald
DOI: 10.1083/jcb.72.2.482
发表时间: 1977-02
期刊: The Journal of cell biology
影响因子: --
作者:
Anderson RG;Hein CE
通讯作者: Hein CE
DOI: 10.3109/00016345609155713
发表时间: 1956
影响因子: 4.3
作者:
U. Borell;O. Nilsson;J. Wersäll;A. Westman
通讯作者: A. Westman
DOI: 10.1002/ar.1091300302
发表时间: 1958
期刊: The Anatomical Record
影响因子: --
作者:
G. S. Greenwald
通讯作者: G. S. Greenwald