Microtubule assembly is required for the formation of the pronuclei, nuclear lamin acquisition, and DNA synthesis during mouse, but not sea urchin, fertilization.
Microtubule assembly is required for the formation of the pronuclei, nuclear lamin acquisition, and DNA synthesis during mouse, but not sea urchin, fertilization.
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小鼠受精过程中原核的形成、核纤层蛋白的获取和 DNA 合成需要微管组装，但海胆则不需要。
DOI：
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
G. Schatten
中科院分区：
文献类型：
--
作者：
H. Schatten;C. Simerly;G. Maul;G. Schatten
在小鼠的受精过程中，雄性和雌性原核的形成需要微管组装，而海胆受精不需要。在小鼠卵母细胞中，50微米秋水仙素在体外受精过程中阻止了母体减数分裂染色体和结合的精子核的解聚。核层不与亲本染色质集合中的任何一个相关联，尽管核外周蛋白(Pl核外周抗原)出现在两者上。在这些受精卵中，DNA合成不会发生。这种影响仅限于排卵后的头几个小时，因为在4-6小时后加入Colcemid不再阻止原核发育、层粘连蛋白的获得或DNA合成。无论是用10微米紫杉醇稳定微管，还是用10微米细胞松弛素D或2.2微克/毫升马兜铃素A抑制微丝，都不能阻止这些原核事件；这些药物将抑制原核在卵中心的定位。在海胆卵中，秋水仙素或灰黄霉素处理不会产生同样的效果，雄性原核的形成伴随着核层的积累。原核形成过程中对微管组装需求的差异可能与细胞周期有关：在小鼠中，精子进入减数分裂的细胞质，而在海胆卵中，精子进入间期细胞质。对有丝分裂的海胆卵进行再受精，以验证这种现象是否与精子进入减数分裂/有丝分裂细胞质或处于间期有关；在第一次有丝分裂的参照过程中，被结合的精子核不能解聚并获得片层。这些结果表明，在小鼠受精过程中，从减数分裂到第一个间期需要微管组装，并表明细胞骨架是受精和细胞周期所必需的核结构变化所必需的。
Microtubule assembly is required for the formation of the male and female pronuclei during mouse, but not sea urchin, fertilization. In mouse oocytes, 50 microM colcemid prevents the decondensation of the maternal meiotic chromosomes and of the incorporated sperm nucleus during in vitro fertilization. Nuclear lamins do not associate with either of the parental chromatin sets although peripherin, the Pl nuclear peripheral antigen, appears on both. DNA synthesis does not occur in these fertilized, colcemid-arrested oocytes. This effect is limited to the first hours after ovulation, since colcemid added 4-6 hours later no longer prevents pronuclear development, lamin acquisition, or DNA synthesis. Neither microtubule stabilization with 10 microM taxol nor microfilament inhibition with 10 microM cytochalasin D or 2.2 micrograms/ml latrunculin A prevent these pronuclear events; these drugs will inhibit the apposition of the pronuclei at the egg center. In sea urchin eggs, colcemid or griseofulvin treatment does not result in the same effect and the male pronucleus forms with the attendant accumulation of the nuclear lamins. The differences in the requirement for microtubule assembly during pronucleus formation may be related to the cell cycle: In mice the sperm enters a meiotic cytoplasm, whereas in sea urchin eggs it enters an interphase cytoplasm. Refertilization of mitotic sea urchin eggs was performed to test the possibility that this phenomenon is related to whether the sperm enters a meiotic/mitotic cytoplasm or one at interphase; during refertilization at first mitosis, the incorporated sperm nucleus is unable to decondense and acquire lamins. These results indicate a requirement for microtubule assembly for the progression from meiosis to first interphase during mouse fertilization and suggest that the cytoskeleton is required for changes in nuclear architecture necessary during fertilization and the cell cycle.
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使用人自身免疫血清检测小鼠、海胆和蛤的卵母细胞核中的核纤层蛋白 B 表位。
影响因子：
2.7
作者：
Maul,GG;Schatten,G;Jimenez,SA;Carrera,AE
通讯作者：
Carrera,AE
DOI：
10.1073/pnas.82.14.4727
发表时间：
1985-01-01
影响因子：
11.1
作者：
SCHATTEN, G;MAUL, GG;BROWN, DL
通讯作者：
BROWN, DL
影响因子：
2.5
作者：
Redel, Bethany K.;Brown, Alana N.;Prather, Randall S.
通讯作者：
Prather, Randall S.
确定海胆受精过程中早期事件的时间。
影响因子：
2.7
作者：
Schatten,G;Hülser,D
通讯作者：
Hülser,D