A novel model of acquired hydrocephalus for evaluation of neurosurgical treatments.
A novel model of acquired hydrocephalus for evaluation of neurosurgical treatments.
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DOI：
10.1186/s12987-021-00281-0
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发表时间：
2021-11-08
影响因子：
7.3
通讯作者：
Limbrick DD Jr
中科院分区：
文献类型：
--
作者：
McAllister JP 2nd;Talcott MR;Isaacs AM;Zwick SH;Garcia-Bonilla M;Castaneyra-Ruiz L;Hartman AL;Dilger RN;Fleming SA;Golden RK;Morales DM;Harris CA;Limbrick DD Jr
许多动物模型已被用于研究脑积水的病理生理学;其中大多数是啮齿动物模型，其无脑大脑皮质可能不会以与脑回物种相同的方式对脑室扩大做出反应，并且其大小不适合临床相关神经外科治疗的评价。脑回物种脑积水的模型较少，因此，我们扩大了幼年猪脑积水的猪模型，并用它来探索手术治疗方法。通过经皮脑池内注射高岭土在33-41日龄的猪中诱导获得性脑积水（n = 17）。对照组包括假盐水注射（n = 6）和完整（n = 4）动物。磁共振成像（MRI）用于评价高岭土给药后11-42天的脑室扩大，并计划高岭土给药后14-38天的脑室腹腔分流术植入手术。评估行为和神经系统状态。诱导后，脑室的所有区域均发生双侧脑室扩大，第三脑室、Monro孔和大脑导水管中存在明显的CSF流空。高岭土沉积物在基底池中形成固体铸件，但枕大池是开放的。在17只未经治疗的脑积水动物中。在11-43日龄时，平均总心室容积为8898 ± 5917 SD mm 3，这显著大于年龄为45-55日龄的6个假手术对照的基线值2251 ± 194 SD mm 3（p < 0.001）。在诱导后恢复期后，未给药猪无症状，尽管表现出轻度-中度脑室扩大。在给药20-30天后，4只分流动物中有3只显示心室容积减少，但一些动物因推定的分流功能障碍而出现共济失调和嗜睡。高岭土诱导幼年猪获得性脑积水产生了一种高度转化的体内模型，允许系统研究脑积水的病理生理学和临床治疗。在线版本包含补充材料，可通过10.1186/s12987-021-00281-0获得。
Many animal models have been used to study the pathophysiology of hydrocephalus; most of these have been rodent models whose lissencephalic cerebral cortex may not respond to ventriculomegaly in the same way as gyrencephalic species and whose size is not amenable to evaluation of clinically relevant neurosurgical treatments. Fewer models of hydrocephalus in gyrencephalic species have been used; thus, we have expanded upon a porcine model of hydrocephalus in juvenile pigs and used it to explore surgical treatment methods. Acquired hydrocephalus was induced in 33–41-day old pigs by percutaneous intracisternal injections of kaolin (n = 17). Controls consisted of sham saline-injected (n = 6) and intact (n = 4) animals. Magnetic resonance imaging (MRI) was employed to evaluate ventriculomegaly at 11–42 days post-kaolin and to plan the surgical implantation of ventriculoperitoneal shunts at 14–38-days post-kaolin. Behavioral and neurological status were assessed. Bilateral ventriculomegaly occurred post-induction in all regions of the cerebral ventricles, with prominent CSF flow voids in the third ventricle, foramina of Monro, and cerebral aqueduct. Kaolin deposits formed a solid cast in the basal cisterns but the cisterna magna was patent. In 17 untreated hydrocephalic animals. Mean total ventricular volume was 8898 ± 5917 SD mm3 at 11–43 days of age, which was significantly larger than the baseline values of 2251 ± 194 SD mm3 for 6 sham controls aged 45–55 days, (p < 0.001). Past the post-induction recovery period, untreated pigs were asymptomatic despite exhibiting mild-moderate ventriculomegaly. Three out of 4 shunted animals showed a reduction in ventricular volume after 20–30 days of treatment, however some developed ataxia and lethargy, from putative shunt malfunction. Kaolin induction of acquired hydrocephalus in juvenile pigs produced an in vivo model that is highly translational, allowing systematic studies of the pathophysiology and clinical treatment of hydrocephalus. The online version contains supplementary material available at 10.1186/s12987-021-00281-0.
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DOI：
10.1098/rspb.1967.0002
发表时间：
1967-01-01
期刊：
PROCEEDINGS OF THE ROYAL SOCIETY SERIES B-BIOLOGICAL SCIENCES
影响因子：
--
作者：
DICKERSON, JW;DOBBING, J
通讯作者：
DOBBING, J
影响因子：
1.9
作者：
Aquilina, Kristian;Chakkarapani, Ela;Thoresen, Marianne
通讯作者：
Thoresen, Marianne
DOI：
10.1007/bf02337439
发表时间：
1990-12-01
期刊：
ITALIAN JOURNAL OF NEUROLOGICAL SCIENCES
影响因子：
--
作者：
DITRAPANI, G;GARZETTI, GG;PENTIMALLI, LCR
通讯作者：
PENTIMALLI, LCR
影响因子：
6.7
作者：
DIGGS, J;PRICE, AC;JAMES, AE
通讯作者：
JAMES, AE
影响因子：
3
作者：
Clancy, Barbara;Kersh, Brandon;Finlay, Barbara L.
通讯作者：
Finlay, Barbara L.