Development of a Mesoamerican intra-genepool genetic map for quantitative trait loci detection in a drought tolerant × susceptible common bean (Phaseolus vulgaris L.) cross.
Development of a Mesoamerican intra-genepool genetic map for quantitative trait loci detection in a drought tolerant × susceptible common bean (Phaseolus vulgaris L.) cross.
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DOI：
10.1007/s11032-010-9527-9
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发表时间：
2012-01
期刊：
影响因子：
--
通讯作者：
Rao IM
中科院分区：
文献类型：
--
作者：
Blair MW;Galeano CH;Tovar E;Muñoz Torres MC;Castrillón AV;Beebe SE;Rao IM
干旱是菜豆（Phaseolus vulgaris L.）生产的主要限制因素，特别是在作物灌溉不频繁的发展中国家。中美洲基因库是常见豆类中种植最广泛的细分类别，包括小型红籽、小型奶油色和黑籽品种。本研究的目的是为中美洲×中美洲耐旱×易感杂交品种开发可靠的遗传图谱，并利用该图谱分析三年实验中干旱和完全灌溉条件下产量性状的遗传。杂交中使用的耐旱来源是奶油籽高级品系 BAT477 与小红色品种 DOR364 杂交，群体由 F5 代重组自交系组成。通过对由两种处理（灌溉和雨养）组成的每个田间环境的总种子产量、每日产量、100粒种子重量、开花天数和成熟天数等性状的复合区间作图来检测数量性状基因座，并在总共六种环境中进行三次重复的网格设计实验。基于扩增片段长度多态性和随机扩增多态性 DNA 标记的遗传图谱锚定有 60 个简单序列重复 (SSR) 标记，图谱总长度为 1,087.5 cM，跨越 11 个连锁群，覆盖整个菜豆基因组，每 5.9 cM 一个标记饱和。遗传图谱的缺口存在于连锁群b03、b09和b11上，但总体而言只有9个缺口大于15 cM。所有性状都是定量遗传的，其中种子重量的数量最多，其次是每日产量、产量本身、开花天数和成熟天数。特别是根据中美洲基因库中作物耐旱性的改良，讨论了这些结果与普通豆类育种的相关性。
Drought is a major constraint to common bean (Phaseolus vulgaris L.) production, especially in developing countries where irrigation for the crop is infrequent. The Mesoamerican genepool is the most widely grown subdivision of common beans that include small red, small cream and black seeded varieties. The objective of this study was to develop a reliable genetic map for a Mesoamerican × Mesoamerican drought tolerant × susceptible cross and to use this map to analyze the inheritance of yield traits under drought and fully irrigated conditions over 3 years of experiments. The source of drought tolerance used in the cross was the cream-seeded advanced line BAT477 crossed with the small red variety DOR364 and the population was made up of recombinant inbred lines in the F5 generation. Quantitative trait loci were detected by composite interval mapping for the traits of overall seed yield, yield per day, 100 seed weight, days to flowering and days to maturity for each field environment consisting of two treatments (irrigated and rainfed) and lattice design experiments with three repetitions for a total of six environments. The genetic map based on amplified fragment length polymorphism and random amplified polymorphic DNA markers was anchored with 60 simple sequence repeat (SSR) markers and had a total map length of 1,087.5 cM across 11 linkage groups covering the whole common bean genome with saturation of one marker every 5.9 cM. Gaps for the genetic map existed on linkage groups b03, b09 and b11 but overall there were only nine gaps larger than 15 cM. All traits were inherited quantitatively, with the greatest number for seed weight followed by yield per day, yield per se, days to flowering and days to maturity. The relevance of these results for breeding common beans is discussed in particular in the light of crop improvement for drought tolerance in the Mesoamerican genepool.
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影响因子：
2.3
作者：
Beebe, S;Rengifo, J;Tohme, J
通讯作者：
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5.4
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