Natural infection of murine norovirus in conventional and specific pathogen-free laboratory mice.
Natural infection of murine norovirus in conventional and specific pathogen-free laboratory mice.
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常规和特异性无病原体实验室小鼠中诺如病毒的自然感染。
DOI：
10.3389/fmicb.2013.00012
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发表时间：
2013
影响因子：
5.2
通讯作者：
Urano T
中科院分区：
文献类型：
--
作者：
Ohsugi T;Matsuura K;Kawabe S;Nakamura N;Kumar JM;Wakamiya M;Morikawa S;Urano T
诺如病毒是全世界大多数急性病毒性肠胃炎病例的病因。由于缺乏人类诺如病毒的细胞培养感染模型，因此需要使用分子方法和/或适合细胞培养的病毒替代模型来预测诺如病毒的失活。小鼠诺如病毒（MNV）是唯一能在细胞培养和小动物模型中复制的诺如病毒，因此可用于构建研究诺如病毒生物学和发病机制的小动物模型。然而，最近的研究表明，自然MNV感染在实验室小鼠群体中广泛存在。我们利用血清学和分子技术研究了来自日本和美国的常规和特异性无病原体（SPF）转基因小鼠以及来自日本几个动物育种商的商业小鼠的MNV感染情况。在67.3%的日本常规小鼠、39.1%的SPF小鼠和62.5%的美国常规小鼠中检测到MNV抗体。在三种育种者之一的20%的商业SPF C57BL/6小鼠中也发现了MNV抗体。对感染动物粪便分离株的部分基因扩增结果表明，分离株与报道的MNV序列同源。这些结果表明，常规和SPF实验室小鼠，包括商业小鼠，都广泛感染了MNV，作为人类疾病的动物模型，这可能需要引起相当大的关注。
Noroviruses cause most cases of acute viral gastroenteritis worldwide. The lack of a cell culture infection model for human norovirus necessitates the use of molecular methods and/or viral surrogate models amenable to cell culture to predict norovirus inactivation. Murine norovirus (MNV) may be used to construct a small animal model for studying the biology and pathogenesis of noroviruses because MNV is the only norovirus that replicates in cell culture and a small animal model. However, recent studies have shown that natural MNV infection is widespread in laboratory mouse colonies. We investigated MNV infection in both conventional and specific pathogen-free (SPF) genetically modified mice from Japan and the US, and commercial mice from several animal breeders in Japan, using serological and molecular techniques. MNV antibodies were detected in 67.3% of conventional mice and 39.1% of SPF mice from Japan and 62.5% of conventional mice from the US. MNV antibodies were also found in 20% of commercial SPF C57BL/6 mice from one of three breeders. Partial gene amplification of fecal isolates from infected animals showed that the isolates were homologous to reported MNV sequences. These results suggest that both conventional and SPF laboratory mice, including commercial mice, are widely infected with MNV, which might require considerable attention as an animal model of human disease.
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影响因子：
2.4
作者：
Goto, Kazuo;Hayashimoto, Nobuhito;Kagiyama, Naoko
通讯作者：
Kagiyama, Naoko
影响因子：
2.4
作者：
Nakamura, N;Yoshizumi, S;Ohsugi, T
通讯作者：
Ohsugi, T
影响因子：
56.9
作者：
Karst, SM;Wobus, CE;Virgin, HW
通讯作者：
Virgin, HW
影响因子：
2.6
作者：
Kitajima, Masaaki;Oka, Tomoichiro;Katayama, Kazuhiko
通讯作者：
Katayama, Kazuhiko
影响因子：
2.7
作者：
Müller B;Klemm U;Mas Marques A;Schreier E
通讯作者：
Schreier E