The role of aspirin and dipyridamole on vascular DNA synthesis and intimal hyperplasia following deendothelialization.
The role of aspirin and dipyridamole on vascular DNA synthesis and intimal hyperplasia following deendothelialization.
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阿司匹林和双嘧达莫对去内皮化后血管 DNA 合成和内膜增生的作用。
DOI：
10.1016/0022-4804(86)90013-2
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Hagen,PO
中科院分区：
文献类型：
--
作者：
Radic,ZS;O'Malley,MK;Mikat,EM;Makhoul,RG;McCann,RL;Cole,CW;Hagen,PO
血小板参与急性血栓形成事件，并通过血小板衍生生长因子参与内膜增生的发展。我们在体内研究了阿司匹林和潘生丁对球囊导管损伤后血管平滑肌细胞增殖和DNA合成的影响。将58只雄性新西兰白色家兔平均分为两组;试验组从手术前2天开始喂食阿司匹林（14 mg/kg/天）和双嘧达莫（9 mg/kg/天），直至损伤后1、2、3、4、7、14或28天处死。所有动物在注射[3 H]胸苷后1 h处死，测定平滑肌细胞DNA比活性和总动力学活性。内膜增生通过光学显微镜测量，内膜核增殖通过计数每毫米内弹性膜的核来确定。核增殖在第14天达到最大值（25 ± 1.2），但内膜增生在第28天仍在增加。24小时后的DNA比活性（试验组：4 ± 2 dpm/μg DNA;对照组：3.3 ± 3 dpm/μg DNA）与未损伤家兔的基础水平相似。两组的DNA合成在第二天和第三天达到峰值（试验组：177 ± 27 dpm/μg DNA;对照组：185 ± 39 dpm/μg DNA），然后缓慢下降至基线值。尽管在胶原蛋白激发（6 μg/ml）后血小板聚集抑制率为90%，[14 C] 5-羟色胺释放显著减少（78%），但治疗组和正常组家兔在[3 H]胸苷掺入、核增殖或内膜增生方面均无显著差异。这些结果表明，兔血管平滑肌中存在另一种显著的生长因子，因为对血小板的全面干扰不会抑制平滑肌增殖。
Platelets are implicated both in acute thrombotic events and, through platelet-derived growth factor, in the development of intimal hyperplasia. We have investigated,in vivo, the influence of aspirin and dipyridamole on vascular smooth muscle cell proliferation and DNA synthesis following balloon catheter injury. Fifty-eight male, New Zealand white rabbits were divided equally into two groups; the test group was fed aspirin (14 mg/kg/day) and dipyridamole (9 mg/kg/day) from 2 days prior to surgery until sacrifice at 1, 2, 3, 4, 7, 14, or 28 days after injury. All animals were sacrificed 1 h after injection of [3H]thymidine and the smooth muscle cell DNA specific activity and total kinetic activity were determined. Intimal hyperplasia was measured by light microscopy and intimal nuclear proliferation was determined by counting nuclei per millimeter of internal elastic lamina. Nuclear proliferation was maximal at 14 days (25 ± 1.2) but intimal hyperplasia was still increasing at 28 days. DNA specific activity after 24 hr (test: 4 ± 2 dpm/μg DNA; control: 3.3 ± 3 dpm/μg DNA) was similar to basal levels in uninjured rabbits. DNA synthesis peaked in both groups between the second and third day (test: 177 ± 27 dpm/μg DNA; control: 185 ± 39 dpm/μg DNA) and then declined slowly toward baseline values. There was no significant difference between treated and normal rabbits in either [3H]thymidine incorporation, nuclear proliferation, or development of intimal hyperplasia despite 90% inhibition of platelet aggregation and a significant reduction (78%) in [14C]serotonin release following collagen challenge (6 μg/ml). These results suggest the presence of another significant growth factor(s) for vascular smooth muscle in the rabbit as global interference with platelets does not inhibit smooth muscle proliferation.
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DOI：
10.1016/0002-9149(81)90254-x
发表时间：
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DOI：
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DOI：
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发表时间：
1986
期刊：
Current surgery
影响因子：
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Lucas3rd,JF;Makhoul,RG;Cole,CW;Mikat,E;McCann,RL;Hagen,PO
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