Nitrate-responsive oral microbiome modulates nitric oxide homeostasis and blood pressure in humans.
Nitrate-responsive oral microbiome modulates nitric oxide homeostasis and blood pressure in humans.
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DOI：
10.1016/j.freeradbiomed.2018.05.078
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发表时间：
2018-08-20
影响因子：
7.4
通讯作者：
Jones AM
中科院分区：
文献类型：
--
作者：
Vanhatalo A;Blackwell JR;L'Heureux JE;Williams DW;Smith A;van der Giezen M;Winyard PG;Kelly J;Jones AM
口腔微生物群落的不平衡与心血管和代谢健康的降低有关。将口腔微生物群与健康联系起来的可能机制是硝酸盐（NO3-）-亚硝酸盐（NO2-）-一氧化氮（NO）途径，该途径依赖于口腔细菌将NO3-还原为NO2-。NO（由NO2-和L-精氨酸产生）调节血管内皮功能，从而调节血压（BP）。通过对细菌16 S rRNA基因进行测序，我们研究了年轻人（18-22岁）和老年人（70-79岁）血压正常的人（n = 18）在补充NO3-（12 mmol/d）和安慰剂10天后，口腔微生物组与NO生物利用度的生理指标之间的关系以及这些变量的可能变化。与安慰剂相比，补充NO3改变了唾液微生物组，增加了变形菌的相对丰度（+225%），降低了拟杆菌的相对丰度（-46%; P < 0.05）。补充NO3后，罗氏菌（+127%）和奈瑟菌（+351%）的相对丰度高于安慰剂条件下的相对丰度，而普氏菌（-60%）和韦荣氏菌（-65%）则低于安慰剂条件下的相对丰度（均P < 0.05）。NO3-补充增加血浆NO2-浓度，降低老年人（70-79岁）的全身血压，但不年轻（18-22岁），参与者。高丰度的罗氏菌和奈瑟氏球菌和低丰度的普雷沃菌和韦荣球菌与更大的增加血浆[NO2-]响应NO3-补充。目前的研究结果表明，口腔微生物组具有可塑性，可以随着无机NO3-饮食摄入量的增加而变化，并且饮食诱导的口腔微生物群落变化与体内NO稳态和血管健康指数有关。膳食硝酸盐改变健康年轻人和老年人的唾液微生物组。硝酸盐的补充降低了普雷沃氏菌和韦荣氏菌的相对丰度。硝酸盐补充增加了罗氏菌和奈瑟菌的相对丰度。唾液微生物组调节一氧化氮生物利用度和血压。
Imbalances in the oral microbial community have been associated with reduced cardiovascular and metabolic health. A possible mechanism linking the oral microbiota to health is the nitrate (NO3-)-nitrite (NO2-)-nitric oxide (NO) pathway, which relies on oral bacteria to reduce NO3- to NO2-. NO (generated from both NO2- and L-arginine) regulates vascular endothelial function and therefore blood pressure (BP). By sequencing bacterial 16S rRNA genes we examined the relationships between the oral microbiome and physiological indices of NO bioavailability and possible changes in these variables following 10 days of NO3- (12 mmol/d) and placebo supplementation in young (18–22 yrs) and old (70–79 yrs) normotensive humans (n = 18). NO3- supplementation altered the salivary microbiome compared to placebo by increasing the relative abundance of Proteobacteria (+225%) and decreasing the relative abundance of Bacteroidetes (−46%; P < 0.05). After NO3-supplementation the relative abundances of Rothia (+127%) and Neisseria (+351%) were greater, and Prevotella (−60%) and Veillonella (−65%) were lower than in the placebo condition (all P < 0.05). NO3- supplementation increased plasma concentration of NO2- and reduced systemic blood pressure in old (70–79 yrs), but not young (18–22 yrs), participants. High abundances of Rothia and Neisseria and low abundances of Prevotella and Veillonella were correlated with greater increases in plasma [NO2-] in response to NO3- supplementation. The current findings indicate that the oral microbiome is malleable to change with increased dietary intake of inorganic NO3-, and that diet-induced changes in the oral microbial community are related to indices of NO homeostasis and vascular health in vivo. Dietary nitrate alters the salivary microbiome of healthy young and old adults. Nitrate supplementation decreases relative abundances of Prevotella and Veillonella. Nitrate supplementation increases relative abundances of Rothia and Neisseria. The salivary microbiome modulates nitric oxide bioavailability and blood pressure.
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DOI：
10.3791/51697
发表时间：
2014-08-27
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
Goode MR;Cheong SY;Li N;Ray WC;Bartlett CW
通讯作者：
Bartlett CW
影响因子：
3.7
作者：
Lima DLF;Carneiro SDRM;Barbosa FTS;Saintrain MVL;Moizan JAH;Doucet J
通讯作者：
Doucet J
影响因子：
3.7
作者：
Biagi E;Nylund L;Candela M;Ostan R;Bucci L;Pini E;Nikkïla J;Monti D;Satokari R;Franceschi C;Brigidi P;De Vos W
通讯作者：
De Vos W
影响因子：
7.4
作者：
Larsen, Filip J.;Weitzberg, Eddie;Ekblom, Bjorn
通讯作者：
Ekblom, Bjorn
影响因子：
7.4
作者：
Burleigh, Mia C.;Liddle, Luke;Easton, Chris
通讯作者：
Easton, Chris