Interleukin 7 from maternal milk crosses the intestinal barrier and modulates T-cell development in offspring.
Interleukin 7 from maternal milk crosses the intestinal barrier and modulates T-cell development in offspring.
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DOI：
10.1371/journal.pone.0020812
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Ngom PT
中科院分区：
文献类型：
--
作者：
Aspinall R;Prentice AM;Ngom PT
母乳喂养可以防止危险环境中的疾病和死亡，这种作用部分是通过改善免疫功能来介导的。一种假设认为，牛奶中的因子可以补充后代不足的免疫反应，但这无法解释一系列观察结果，这些观察结果表明，母乳中的因子可能通过对初级淋巴器官的直接影响来补充免疫系统的发育。在之前的一项人类研究中，我们报告了证据表明母乳中的 IL-7 与婴儿的胸腺输出之间存在联系。在这里，我们报告了小鼠中母源性 IL-7 对后代 T 细胞发育有直接作用的证据。我们使用荧光染料标记的重组 IL-7 来追踪活体小鼠中 IL-7 从胃穿过肠道进入淋巴组织的运动。为了验证母体来源的 IL-7 的功能能力，我们将 IL-7 敲除小鼠与正常野生型母体杂交培养。胸腺细胞亚群和外周 T 细胞群显着高于接受 IL-7 敲除母亲乳汁的敲除小鼠。我们的研究提供了直接证据，表明白介素 7（一种对 T 淋巴细胞发育至关重要的因子），当母体来源时，可以穿过后代的肠道，增加胸腺中 T 细胞的产生，并支持外周次级淋巴组织中 T 细胞的存活。
Breastfeeding protects against illnesses and death in hazardous environments, an effect partly mediated by improved immune function. One hypothesis suggests that factors within milk supplement the inadequate immune response of the offspring, but this has not been able to account for a series of observations showing that factors within maternally derived milk may supplement the development of the immune system through a direct effect on the primary lymphoid organs. In a previous human study we reported evidence suggesting a link between IL-7 in breast milk and the thymic output of infants. Here we report evidence in mice of direct action of maternally-derived IL-7 on T cell development in the offspring. We have used recombinant IL-7 labelled with a fluorescent dye to trace the movement in live mice of IL-7 from the stomach across the gut and into the lymphoid tissues. To validate the functional ability of maternally derived IL-7 we cross fostered IL-7 knock-out mice onto normal wild type mothers. Subsets of thymocytes and populations of peripheral T cells were significantly higher than those found in knock-out mice receiving milk from IL-7 knock-out mothers. Our study provides direct evidence that interleukin 7, a factor which is critical in the development of T lymphocytes, when maternally derived can transfer across the intestine of the offspring, increase T cell production in the thymus and support the survival of T cells in the peripheral secondary lymphoid tissue.
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