Potential Antagonistic Bacteria against Verticillium dahliae Isolated from Artificially Infested Nursery.
Potential Antagonistic Bacteria against Verticillium dahliae Isolated from Artificially Infested Nursery.
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DOI：
10.3390/cells10123588
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发表时间：
2021-12-20
期刊：
影响因子：
6
通讯作者：
Cheng H
中科院分区：
文献类型：
--
作者：
Su X;Wu S;Liu L;Lu G;Liu H;Jin X;Wang Y;Guo H;Wang C;Cheng H
拮抗细菌作为一种生态友好的生防菌，是田间防治棉花黄萎病的一类重要的高效杀菌剂。黄萎病是棉花等作物上最强的病原菌。为了确定急需的候选细菌，我们在人工侵染的苗圃中筛选了从棉花根际土壤中分离的细菌对大丽弧菌的拮抗活性。在拮抗候选菌株的初步试验中，以芽孢杆菌为主的88个菌株对大丽弧菌的菌落直径有较强的抑制作用，其中9个菌株的抑制率高达50%。在最有效的细菌菌株中，芽孢杆菌。ABLF-18、ABLF-50和拟杆菌属(Paenibacillussp.)ABLF-90显著降低了棉花的病情指数和真菌生物量，达到对照的40-70%。在进一步的生防试验中(S)，菌株分泌了能降解菌丝的纤维素酶、葡聚糖酶和蛋白酶，以及表面素和伊图林同系物等抗菌脂肽。与抗病相关的PAL、MAPK和PR10基因也在棉花植株中诱导表达。结果表明，三个候选菌株均能增强棉花对大丽叶病毒的防御反应；真菌细胞壁降解酶的分泌、非核糖体抗菌肽的合成和系统抗性的诱导表明，这些菌株具有很大的生防杀菌剂潜力。
As an ecofriendly biocontrol agent, antagonistic bacteria are a crucial class of highly efficient fungicides in the field against Verticillium dahliae, the most virulent pathogen for cotton and other crops. Toward identifying urgently needed bacterial candidates, we screened bacteria isolated from the cotton rhizosphere soil for antagonisitic activity against V. dahliae in an artificially infested nursery. In preliminary tests of antagonistic candidates to characterize the mechanism of action of on culture medium, 88 strains that mainly belonged to Bacillus strongly inhibited the colony diameter of V. dahliae, with inhibiting efficacy up to 50% in 9 strains. Among the most-effective bacterial strains, Bacillus sp. ABLF-18, and ABLF-50 and Paenibacillus sp. ABLF-90 significantly reduced the disease index and fungal biomass of cotton to 40–70% that of the control. In further tests to elucidate the biocontrol mechanism (s), the strains secreted extracellular enzymes cellulase, glucanase, and protease, which can degrade the mycelium, and antimicrobial lipopeptides such as surfactin and iturin homologues. The expression of PAL, MAPK and PR10, genes related to disease resistance, was also elicited in cotton plants. Our results clearly show that three candidate bacterial strains can enhance cotton defense responses against V. dahliae; the secretion of fungal cell-wall-degrading enzymes, synthesis of nonribosomal antimicrobial peptides and induction of systemic resistance shows that the strains have great potential as biocontrol fungicides.
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DOI：
10.1155/2011/824104
发表时间：
2011
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Cao H;He S;Wei R;Diong M;Lu L
通讯作者：
Lu L
影响因子：
14.9
作者：
Flissi, Areski;Ricart, Emma;Pupin, Maude
通讯作者：
Pupin, Maude
影响因子：
6.7
作者：
Choudhary, Devendra K.;Johri, Bhavdish N.
通讯作者：
Johri, Bhavdish N.
影响因子：
4.4
作者：
Asaka, O;Shoda, M
通讯作者：
Shoda, M
影响因子：
4.2
作者：
Erdogan, Oktay;Benlioglu, Kemal
通讯作者：
Benlioglu, Kemal