The extra-embryonic area opaca plays a role in positioning the primitive streak of the early chick embryo.

The extra-embryonic area opaca plays a role in positioning the primitive streak of the early chick embryo.
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DOI:
10.1242/dev.200303
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发表时间:
2022-06-15
期刊:
Development (Cambridge, England)
影响因子:
--
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--
中科院分区:
其他
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经典的研究已经确定,边缘区,一个环的胚外上胚层直接围绕鸡胚的胚胎上胚层(透明区),是重要的设置胚胎极性定位原条,原肠胚形成的网站。更外部的胚外区域(区域opaca)被认为只有营养和支持功能。使用实验胚胎学方法,这项研究揭示了三个可分离的功能,这个外部区域。首先,并置区opaca直接到透明区诱导一个新的边缘区从后者,这个诱导域是完全后的性质。第二,消融和移植实验,使用一个孤立的前半部分的胚盘和件的区域opaca表明,该地区opaca可以影响相邻的边缘区的极性。最后,我们表明,这种孤立的前半胚胎的能力,以调节(重新建立极性自发)在早期原始条纹阶段的损失,可以获救,由一个年轻的阶段取代该地区opaca。这些结果揭示了鸡胚外组织在早期发育中的新作用。总结:两个相邻的胚外组织,不透明区和边缘区,相互作用,影响早期鸡胚的极性。
Classical studies have established that the marginal zone, a ring of extra-embryonic epiblast immediately surrounding the embryonic epiblast (area pellucida) of the chick embryo, is important in setting embryonic polarity by positioning the primitive streak, the site of gastrulation. The more external extra-embryonic region (area opaca) was thought to have only nutritive and support functions. Using experimental embryology approaches, this study reveals three separable functions for this outer region. First, juxtaposition of the area opaca directly onto the area pellucida induces a new marginal zone from the latter; this induced domain is entirely posterior in character. Second, ablation and grafting experiments using an isolated anterior half of the blastoderm and pieces of area opaca suggest that the area opaca can influence the polarity of the adjacent marginal zone. Finally, we show that the loss of the ability of such isolated anterior half-embryos to regulate (re-establish polarity spontaneously) at the early primitive streak stage can be rescued by replacing the area opaca by one from a younger stage. These results uncover new roles of chick extra-embryonic tissues in early development. Summary: Two adjacent extra-embryonic tissues, the area opaca and the marginal zone, interact to influence the polarity of the early chick embryo.
DOI: 10.1242/dev.081901
发表时间: 2012-11
期刊: Development (Cambridge, England)
影响因子: --
作者:
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