Melanophore multinucleation pathways in zebrafish
Melanophore multinucleation pathways in zebrafish
复制标题
斑马鱼黑色素细胞多核途径
DOI：
10.1111/dgd.12564
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Watanabe M
中科院分区：
文献类型：
--
作者：
Usui Y;Kondo S;Watanabe M
在斑马鱼中，除了单核黑色素细胞外，还经常观察到双核和三核黑色素细胞;然而，这些细胞在鱼类发育过程中发生多核化的方式仍然未知。在这里，我们分析了斑马鱼黑色素细胞多核化的过程。转基因斑马鱼中的黑色素细胞核用组蛋白H2 B-红色荧光报告蛋白标记，用于评估躯干和鳍中单核、双核和三核黑色素细胞的分布。检查的黑色素细胞中有一半是双核的，大约1%是三核的。我们比较了在培养皿中生长的单核和双核黑色素细胞之间的细胞大小，细胞运动性和存活率，我们发现双核黑色素细胞的细胞大小和存活率显着更大。然后，我们使用体内和体外活细胞成像分析了转基因斑马鱼的成黑素细胞和黑素细胞的行为。我们检测到分裂和分化的黑素细胞，以及黑素细胞核分裂没有随后的细胞分裂。此外，我们观察到黑色素细胞的细胞和核分裂，虽然这些事件是非常罕见的体外。在我们的研究结果的基础上，我们提出了一个计划，在斑马鱼的黑色素细胞多核。
In zebrafish, apart from mononuclear melanophores, bi‐ and trinuclear melanophores are frequently observed; however, the manner in which multinucleation of these cells occurs during fish development remains unknown. Here, we analyzed the processes underlying multinucleation of zebrafish melanophores. Transgenic zebrafish in which melanophore nuclei were labeled with a histone H2B‐red fluorescent reporter protein were used to evaluate the distribution of mono‐, bi‐, and trinuclear melanophores in both the trunk and fin. Half of the melanophores examined were binuclear and approximately 1% were trinuclear. We compared cell size, cell motility, and survival rate between mono‐ and binuclear melanophores grown in a culture dish, and we found that cell size and survival rate were significantly larger in binuclear melanophores. We then analyzed the behavior of melanoblasts and melanophores from transgenic zebrafish using in vivo and in vitro live‐cell imaging. We detected division and differentiation of melanoblasts, as well as melanoblast nuclear division without subsequent cellular division. In addition, we observed cellular and nuclear division in melanophores, although these events were very infrequent in vitro. On the basis of our findings, we present a scheme for melanophore multinucleation in zebrafish.
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