LAT1 Protein Content Increases Following 12 Weeks of Resistance Exercise Training in Human Skeletal Muscle.
LAT1 Protein Content Increases Following 12 Weeks of Resistance Exercise Training in Human Skeletal Muscle.
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DOI：
10.3389/fnut.2020.628405
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发表时间：
2020
影响因子：
5
通讯作者：
Roberts MD
中科院分区：
文献类型：
--
作者：
Roberson PA;Mobley CB;Romero MA;Haun CT;Osburn SC;Mumford PW;Vann CG;Greer RA;Ferrando AA;Roberts MD
氨基酸转运蛋白是细胞内氨基酸转运和促进蛋白质合成所必需的。虽然之前的文献已经证明了急性抗阻运动和氨基酸补充后氨基酸转运蛋白与蛋白质合成的关联，但抗阻运动和补充对氨基酸转运蛋白的慢性影响尚不清楚。本文的目的是确定氨基酸转运蛋白和氨基酸代谢酶是否与不同营养补充策略的阻力运动训练后骨骼肌肥大有关。方法：将43名大学年龄男性分为麦芽糖糊精安慰剂组（PLA, n = 12）、亮氨酸组（LEU, n = 14）和乳清浓缩蛋白组（WPC, n = 17），进行为期12周的全身阻力运动训练。各组补充总能量和脂肪标准化，LEU和WPC补充总亮氨酸标准化（6 g/d）。在训练前和最后一次训练后72小时进行骨骼肌活检。结果：各组训练后I型和II型纤维横截面积（fCSA）均增加（p < 0.050）。训练后LAT1蛋白升高（p < 0.001）， PLA的升高幅度大于LEU和WPC （p < 0.050）。训练后BCKDHα蛋白升高，ATF4蛋白降低（p < 0.001）。免疫组织化学显示，总LAT1/纤维增加，而膜LAT1/纤维不增加（p = 0.003）。在所有组中，ATF4蛋白的变化与fCSA的变化有相关性，而其他标志物的变化与fCSA的变化无相关性（r = 0.314; p = 0.055）；然而，当使用回归分析来划分组时，ATF4蛋白的变化仅在LEU中最能预测fCSA的变化（r2 = 0.322; p = 0.043）。在C2C12成肌细胞中，LAT1蛋白过表达引起了蛋白质合成水平的降低（p = 0.002）和BCKDHα蛋白的降低（p = 0.001）。结论：氨基酸转运蛋白和代谢酶受到阻力运动训练的影响，但似乎并不决定肌纤维肥大。事实上，在体外过表达LAT1会降低蛋白质合成。
Introduction: Amino acid transporters are essential for cellular amino acid transport and promoting protein synthesis. While previous literature has demonstrated the association of amino acid transporters and protein synthesis following acute resistance exercise and amino acid supplementation, the chronic effect of resistance exercise and supplementation on amino acid transporters is unknown. The purpose herein was to determine if amino acid transporters and amino acid metabolic enzymes were related to skeletal muscle hypertrophy following resistance exercise training with different nutritional supplementation strategies. Methods: 43 college-aged males were separated into a maltodextrin placebo (PLA, n = 12), leucine (LEU, n = 14), or whey protein concentrate (WPC, n = 17) group and underwent 12 weeks of total-body resistance exercise training. Each group's supplement was standardized for total energy and fat, and LEU and WPC supplements were standardized for total leucine (6 g/d). Skeletal muscle biopsies were obtained prior to training and ~72 h following each subject's last training session. Results: All groups increased type I and II fiber cross-sectional area (fCSA) following training (p < 0.050). LAT1 protein increased following training (p < 0.001) and increased more in PLA than LEU and WPC (p < 0.050). BCKDHα protein increased and ATF4 protein decreased following training (p < 0.001). Immunohistochemistry indicated total LAT1/fiber, but not membrane LAT1/fiber, increased with training (p = 0.003). Utilizing all groups, the change in ATF4 protein, but no other marker, trended to correlate with the change in fCSA (r = 0.314; p = 0.055); however, when regression analysis was used to delineate groups, the change in ATF4 protein best predicted the change in fCSA only in LEU (r2 = 0.322; p = 0.043). In C2C12 myoblasts, LAT1 protein overexpression caused a paradoxical decrease in protein synthesis levels (p = 0.002) and decrease in BCKDHα protein (p = 0.001). Conclusions: Amino acid transporters and metabolic enzymes are affected by resistance exercise training, but do not appear to dictate muscle fiber hypertrophy. In fact, overexpression of LAT1 in vitro decreased protein synthesis.
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影响因子：
12.3
作者：
Carpenter AE;Jones TR;Lamprecht MR;Clarke C;Kang IH;Friman O;Guertin DA;Chang JH;Lindquist RA;Moffat J;Golland P;Sabatini DM
通讯作者：
Sabatini DM
DOI：
10.1016/j.biocel.2013.06.011
发表时间：
2013-10
影响因子：
4
作者：
Bodine, Sue C.
通讯作者：
Bodine, Sue C.
DOI：
10.1152/ajpendo.00413.2004
发表时间：
2005-04-01
影响因子：
5.1
作者：
Koopman, R;Wagenmakers, AJM;van Loon, LJC
通讯作者：
van Loon, LJC
DOI：
10.1152/ajpendo.00603.2011
发表时间：
2012-05-01
影响因子：
5.1
作者：
Drummond, Micah J.;Dickinson, Jared M.;Volpi, Elena
通讯作者：
Volpi, Elena
DOI：
10.1152/ajpendo.00582.2007
发表时间：
2008-02-01
影响因子：
5.1
作者：
Dreyer, Hans C.;Drummond, Micah J.;Rasmussen, Blake B.
通讯作者：
Rasmussen, Blake B.