Integrated omics reveal novel functions and underlying mechanisms of the receptor kinase FERONIA in Arabidopsis thaliana.-猫眼课题宝

DOI：

10.1093/plcell/koac111

发表时间：

2022-07-04

期刊：

The Plant cell

影响因子：

通讯作者：

中科院分区：

其他

文献类型：

作者：

来源链接：

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9252503/

受体激酶FERONIA（FER）是植物生长发育、生物和非生物胁迫反应以及繁殖的多功能调节剂。为了获得这些过程的分子相互作用的新见解，并确定新的FER功能，我们进行了定量转录组，蛋白质组和磷酸化蛋白质组分析的拟南芥（拟南芥）野生型和fer-4功能丧失突变体植物。植物激素信号传导，非生物胁迫和生物胁迫的基因本体论术语显着丰富的差异表达的转录本，差异丰富的蛋白质，和/或错误磷酸化的蛋白质，在协议中的FER在这些过程中的已知作用。多组学数据分析和随后的实验证据揭示了FER在内质网（ER）体形成和芥子油苷生物合成中以前未知的功能。FER通过转录因子NAI 1介导ER体的形成。FER也负调控吲哚硫代葡萄糖苷的生物合成，部分通过NAI 1。此外，我们发现一组脱落酸（阿坝）诱导的转录因子在fer-4突变体中被低磷酸化，并证明FER通过转录因子阿坝insensitive 5（ABI 5）负调控子叶绿化过程中的阿坝反应。因此，我们的综合组学研究揭示了FER的新功能，并为FER功能的潜在机制提供了新的见解。多组学数据分析预测了FERONIA在ER体形成和硫代葡萄糖苷生物合成中的新功能，以及FERONIA在阿坝信号传导中对ABI 5的调节。

The receptor kinase FERONIA (FER) is a versatile regulator of plant growth and development, biotic and abiotic stress responses, and reproduction. To gain new insights into the molecular interplay of these processes and to identify new FER functions, we carried out quantitative transcriptome, proteome, and phosphoproteome profiling of Arabidopsis (Arabidopsis thaliana) wild-type and fer-4 loss-of-function mutant plants. Gene ontology terms for phytohormone signaling, abiotic stress, and biotic stress were significantly enriched among differentially expressed transcripts, differentially abundant proteins, and/or misphosphorylated proteins, in agreement with the known roles for FER in these processes. Analysis of multiomics data and subsequent experimental evidence revealed previously unknown functions for FER in endoplasmic reticulum (ER) body formation and glucosinolate biosynthesis. FER functions through the transcription factor NAI1 to mediate ER body formation. FER also negatively regulates indole glucosinolate biosynthesis, partially through NAI1. Furthermore, we found that a group of abscisic acid (ABA)-induced transcription factors is hypophosphorylated in the fer-4 mutant and demonstrated that FER acts through the transcription factor ABA INSENSITIVE5 (ABI5) to negatively regulate the ABA response during cotyledon greening. Our integrated omics study, therefore, reveals novel functions for FER and provides new insights into the underlying mechanisms of FER function. Multi-omics data analysis predicted novel functions of FERONIA in ER body formation and glucosinolate biosynthesis, as well as FERONIA regulation of ABI5 in ABA signaling.