Expression features of SOX9 associate with tumor progression and poor prognosis of hepatocellular carcinoma.
Expression features of SOX9 associate with tumor progression and poor prognosis of hepatocellular carcinoma.
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SOX9的表达特征与肝细胞癌的肿瘤进展和不良预后相关
DOI：
10.1186/1746-1596-7-44
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发表时间：
2012-04-19
影响因子：
2.6
通讯作者：
Zhao J
中科院分区：
文献类型：
--
作者：
Guo X;Xiong L;Sun T;Peng R;Zou L;Zhu H;Zhang J;Li H;Zhao J
sox9作为SOX （SRY[性别决定区Y] box]）基因超家族的成员，先前已被证明是多种恶性肿瘤的原癌基因。然而，SOX9在肝细胞癌（HCC）中表达的临床意义尚不清楚。本研究旨在探讨SOX9在HCC中的表达及其与肿瘤进展和预后的关系。方法选择130例行根治性肝切除术的HCC患者，采用免疫组织化学、Western blotting和定量实时聚合酶链反应（Q-PCR）检测SOX9在各自肿瘤中的表达。结果免疫组织化学、Western blotting和Q-PCR一致证实，与邻近的非肿瘤组织相比，HCC组织中SOX9过表达（P≪0.01）。免疫染色结果显示，高分期（T3 ~ 4）和肿瘤分级（G3）的SOX9阳性细胞多于低分期（T1 ~ 2, P = 0.03）和肿瘤分级（G1 ~ 2, P = 0.01）。此外，SOX9高表达的HCC患者分别与较低的5年总体生存率（P≪0.01）和较低的5年无病生存率（P≪0.01）显著相关。Cox比例风险模型进一步显示，SOX9过表达是HCC患者5年无病生存（风险比[HR] = 2.621, 95%可信区间[CI] = 1.548 ~ 5.829, P = 0.01）和5年总生存（HR = 3.825, CI = 1.638 ~ 7.612, P = 0.003）的独立不良预后因素。结论我们的数据首次提示肝癌组织中SOX9蛋白的过表达对肿瘤进展和预后不良具有预测价值。虚拟幻灯片本文的虚拟幻灯片可以在这里找到：http://www.diagnosticpathology.diagnomx.eu/vs/9029740396926377。
BackgroundSOX9 as a member of the SOX (SRY [sex determining region Y] box) gene superfamily has been previously demonstrated to be a proto-oncogene in a variety of malignancies. However, the clinical significance of SOX9 expression in hepatocellular carcinoma (HCC) remains unclear. The aim of this study was to investigate the expression of SOX9 in HCC and determine its correlation with tumor progression and prognosis.MethodsOne-hundred and thirty HCC patients who had undergone curative liver resection were selected and immunohistochemistry, Western blotting, and quantitative real time polymerase chain reaction (Q-PCR) were performed to analyze SOX9 expression in the respective tumors.ResultsImmunohistochemistry, Western blotting, and Q-PCR consistently confirmed SOX9 overexpression in HCC tissues compared with their adjacent nonneoplastic tissues (P ≪ 0.01). Additionally, immunostaining showed more SOX9 positive cells in the higher tumor stage (T3 ~ 4) and tumor grade (G3) than in the lower tumor stage (T1 ~ 2, P = 0.03) and tumor grade (G1 ~ 2, P = 0.01), respectively. Moreover, HCC patients with high SOX9 expression were significantly associated with lower 5-year overall survival (P ≪ 0.01) and lower 5-year disease-free survival (P ≪ 0.01), respectively. The Cox proportional hazards model further showed that SOX9 over-expression was an independent poor prognostic factor for both 5-year disease-free survival (hazards ratio [HR] = 2.621, 95% confidence interval[CI] = 1.548-5.829, P = 0.01) and 5-year overall survival (HR = 3.825, CI = 1.638-7.612, P = 0.003) in HCC.ConclusionOur data suggest for the first time that the overexpression of SOX9 protein in HCC tissues is of predictive value on tumor progression and poor prognosis.Virtual slidesThe virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/9029740396926377 .
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DOI：
10.1083/jcb.200311021
发表时间：
2004-07-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Blache P;van de Wetering M;Duluc I;Domon C;Berta P;Freund JN;Clevers H;Jay P
通讯作者：
Jay P
影响因子：
6.2
作者：
Xu, Michelle Z.;Yao, Tzy-Jyun;Lee, Nikki P. Y.;Ng, Irene O. L.;Chan, Yuk-Tat;Zender, Lars;Lowe, Scott W.;Poon, Ronnie T. P.;Luk, John M.
通讯作者：
Luk, John M.
影响因子：
10.3
作者：
Krahl, D.;Sellheyer, K.
通讯作者：
Sellheyer, K.
影响因子：
11.2
作者：
Wang, Hongyun;Leav, Irwin;Yuan, Xin
通讯作者：
Yuan, Xin
DOI：
10.1073/pnas.111092198
发表时间：
2001-06-05
影响因子：
11.1
作者：
Bi, WM;Huang, WD;de Crombrugghe, B
通讯作者：
de Crombrugghe, B