Powdery mildew resistance in tomato by impairment of SlPMR4 and SlDMR1.
Powdery mildew resistance in tomato by impairment of SlPMR4 and SlDMR1.
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DOI：
10.1371/journal.pone.0067467
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Bai Y
中科院分区：
文献类型：
--
作者：
Huibers RP;Loonen AE;Gao D;Van den Ackerveken G;Visser RG;Bai Y
拟南芥对白粉病和霜霉病的易感性的遗传解剖已经确定了多个易感性（S）基因，其损伤导致抗病性。虽然这些S基因中的几个已经被克隆并更详细地表征，但它们在疾病易感性中的功能在不同植物物种中保守到何种程度尚不清楚。此外，目前还不清楚这些基因的损伤是否具有抗病育种的潜力，由于可能的健身成本与受损的等位基因。在这里，我们表明，拟南芥PMR 4和DMR 1，基因编码的胼胝质合成酶和高丝氨酸激酶，分别在番茄中的功能直系同源物就其S-基因的功能。使用RNAi沉默这两个基因导致对番茄白粉病真菌新番茄粉孢（Oidium neolycopersici）的抗性。对O.通过SlDMR 1沉默导致的新番茄枯萎病与严重降低的植物生长相关，而SlPMR 4沉默则不相关。因此，SlPMR 4是一个合适的候选基因作为诱变的目标，以获得等位基因，可以部署在番茄抗病育种。
Genetic dissection of disease susceptibility in Arabidopsis to powdery and downy mildew has identified multiple susceptibility (S) genes whose impairment results in disease resistance. Although several of these S-genes have been cloned and characterized in more detail it is unknown to which degree their function in disease susceptibility is conserved among different plant species. Moreover, it is unclear whether impairment of such genes has potential in disease resistance breeding due to possible fitness costs associated with impaired alleles. Here we show that the Arabidopsis PMR4 and DMR1, genes encoding a callose synthase and homoserine kinase respectively, have functional orthologs in tomato with respect to their S-gene function. Silencing of both genes using RNAi resulted in resistance to the tomato powdery mildew fungus Oidium neolycopersici. Resistance to O. neolycopersici by SlDMR1 silencing was associated with severely reduced plant growth whereas SlPMR4 silencing was not. SlPMR4 is therefore a suitable candidate gene as target for mutagenesis to obtain alleles that can be deployed in disease resistance breeding of tomato.
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DOI：
10.1007/s11032-009-9323-6
发表时间：
2010-01
期刊：
Molecular breeding : new strategies in plant improvement
影响因子：
--
作者：
Pavan S;Jacobsen E;Visser RG;Bai Y
通讯作者：
Bai Y
影响因子：
30.8
作者：
Consonni, Chiara;Humphry, Matthew E.;Panstruga, Ralph
通讯作者：
Panstruga, Ralph
影响因子：
11.6
作者：
Stuttmann, Johannes;Hubberten, Hans-Michael;Parker, Jane E.
通讯作者：
Parker, Jane E.
影响因子：
64.8
作者：
Shukla, Vipula K.;Doyon, Yannick;Urnov, Fyodor D.
通讯作者：
Urnov, Fyodor D.
耕作：功能基因组学的快捷方式。
影响因子：
2.4
作者：
Kurowska, Marzena;Daszkowska-Golec, Agata;Gruszka, Damian;Marzec, Marek;Szurman, Miriam;Szarejko, Iwona;Maluszynski, Miroslaw
通讯作者：
Maluszynski, Miroslaw