Interplay between phosphorylation and SUMOylation events determines CESTA protein fate in brassinosteroid signalling.
Interplay between phosphorylation and SUMOylation events determines CESTA protein fate in brassinosteroid signalling.
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DOI：
10.1038/ncomms5687
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发表时间：
2014-08-19
影响因子：
16.6
通讯作者：
Poppenberger, Brigitte
中科院分区：
文献类型：
--
作者：
Khan, Mamoona;Rozhon, Wilfried;Unterholzner, Simon Josef;Chen, Tingting;Eremina, Marina;Wurzinger, Bernhard;Bachmair, Andreas;Teige, Markus;Sieberer, Tobias;Isono, Erika;Poppenberger, Brigitte
油菜素内酯是植物生长所必需的类固醇激素。对这些激素的应答是由BES1/BZR1亚家族的转录因子介导的，油菜素内酯通过破坏GSK3/毛茸茸样激酶对这些因子的抑制磷酸化来激活这些因子。本研究表明，油菜素内酯诱导了CESTA （CES）的核区隔化，CESTA是一种调节油菜素内酯反应的bHLH转录因子，并揭示了这一过程受到CES SUMOylation的调节。我们证明了CES包含一个扩展的SUMOylation基序，并且该基序的SUMOylation被磷酸化拮抗以控制CES亚核定位。此外，我们提供的证据表明，磷酸化调节CES的转录活性和蛋白酶体的蛋白质周转。本文提出了一种协调修饰模型，在油菜素类固醇缺乏的情况下，CES被磷酸化以激活靶基因转录，并实现进一步的翻译后修饰，从而控制CES蛋白的稳定性。
Brassinosteroids are steroid hormones that are essential for plant growth. Responses to these hormones are mediated by transcription factors of the BES1/BZR1 subfamily, and brassinosteroids activate these factors by impairing their inhibitory phosphorylation by GSK3/shaggy-like kinases. Here we show that brassinosteroids induce nuclear compartmentalization of CESTA (CES), a bHLH transcription factor that regulates brassinosteroid responses, and reveal that this process is regulated by CES SUMOylation. We demonstrate that CES contains an extended SUMOylation motif, and that SUMOylation of this motif is antagonized by phosphorylation to control CES subnuclear localization. Moreover, we provide evidence that phosphorylation regulates CES transcriptional activity and protein turnover by the proteasome. A coordinated modification model is proposed in which, in a brassinosteroid-deficient situation, CES is phosphorylated to activate target gene transcription and enable further posttranslational modification that controls CES protein stability.
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