Supragingival mycobiome of HIV-exposed-but-uninfected children reflects a stronger correlation with caries-free-associated taxa compared to HIV-infected or uninfected children.
Supragingival mycobiome of HIV-exposed-but-uninfected children reflects a stronger correlation with caries-free-associated taxa compared to HIV-infected or uninfected children.
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DOI：
10.1128/spectrum.01491-23
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发表时间：
2023-12-12
影响因子：
3.7
通讯作者：
Richards, Vincent P.
中科院分区：
文献类型：
--
作者：
O'Connell, Lauren M.;Mann, Allison E.;Osagie, Esosa;Akhigbe, Paul;Blouin, Thomas;Soule, Ashlyn;Obuekwe, Ozoemene;Omoigberale, Augustine;Burne, Robert A.;Coker, Modupe O.;Richards, Vincent P.
高效抗逆转录病毒治疗（HAART）大大减少了艾滋病毒感染者的机会性感染。然而，即使使用高效抗逆转录病毒疗法，艾滋病毒感染者患口腔疾病（包括龋齿）的风险仍然增加。龋齿的发展与微生物群落的变化有关，导致群落生态失调。HIV感染可以显著改变口腔中的细菌组和真菌组组成;然而，这些影响尚未被评估为龋齿的发生和发展。本研究的目的是描述感染HIV（HI）、暴露HIV但未感染HIV（HEU）和未暴露HIV但未感染HIV（HUU）的儿童（有龋和无龋）龈上菌斑样本的真菌群落特征。为了实现这一点，对127个样品的ITS 1扩增子进行测序。我们发现，HIV感染和暴露导致健康和龋齿的龈上菌斑菌群发生变化。总体而言，减少社区的多样性，龋齿的进展进行了观察，HI儿童的多样性最低，高浓缩铀儿童最高。白色念珠菌是鉴定的最丰富的菌种，具有177种不同的扩增子序列变体（ASV）。两个C。白色念珠菌ASV占优势（占所有分类归属的53.0%和38.5%）。更频繁的ASV占主导地位的HI和HUU社区，而第二占主导地位的高浓缩铀社区。高浓缩铀儿童的C丰度也最低。白色念珠菌和最高数量的健康相关分类群（与无龋牙齿统计相关的分类群）。在全球范围内，龋齿是最常见的慢性儿童疾病之一，尽管有证据表明真菌有助于增加酸的产生，加剧釉质脱矿，但对微生物群落中负责的真菌成分的研究很少。艾滋病毒感染是另一个全球健康危机。围产期HIV暴露与感染是龋齿的危险因素，然而，龋齿的经验，在围产期HIV暴露无感染的情况下是不太清楚。使用高通量扩增子测序，我们发现分类学差异，在后期龋齿变得明显。值得注意的是，我们显示了一个更强的相关性与健康相关的分类为艾滋病毒暴露，但未感染的儿童相比，未暴露和未感染的儿童。这与暴露但未感染的儿童在6岁或以下乳牙中龋齿的发病率较低相一致。最终，这些发现可能有助于改善风险评估，干预和预防策略，如生物膜破坏和知情设计的亲，前和合生素口服疗法。
Highly active antiretroviral treatment (HAART) has greatly reduced opportunistic infections in HIV-infected individuals. However, even with the use of HAART, HIV-infected individuals are still at an increased risk of oral diseases, including dental caries. Dental caries development is associated with microbial community shifts leading to community dysbiosis. HIV infection can significantly alter the bacteriome and mycobiome composition in the oral cavity; however, these impacts have not been assessed for caries initiation and progression. The aim of this study was to characterize the mycobiome for supragingival plaque samples from HIV-infected (HI), HIV-exposed-but-uninfected (HEU), and HIV-unexposed-and-uninfected (HUU) children with and without caries. To accomplish this, ITS1 amplicons of 127 samples were sequenced. We found that HIV infection and exposure resulted in changes to the supragingival plaque mycobiome for both health and caries. Overall, a reduction in community diversity as caries progressed was observed, with HI children having the lowest diversity and HEU children the highest. Candida albicans was the most abundant species identified, with 177 different amplicon sequence variants (ASVs). Two C. albicans ASVs dominated the data (53.0% and 38.5% of all taxonomic assignments). The more frequent ASV dominated HI and HUU communities, whereas the second dominated HEU communities. HEU children also had the lowest abundance of C. albicans and the highest number of health-associated taxa (taxa statistically associated with caries-free teeth). Globally, caries is among the most frequent chronic childhood disease, and the fungal component of the microbial community responsible is poorly studied despite evidence that fungi contribute to increased acid production exacerbating enamel demineralization. HIV infection is another global health crisis. Perinatal HIV exposure with infection are caries risk factors; however, the caries experience in the context of perinatal HIV exposure without infection is less clear. Using high-throughput amplicon sequencing, we find taxonomic differences that become pronounced during late-stage caries. Notably, we show a stronger correlation with health-associated taxa for HIV-exposed-but-uninfected children when compared to unexposed and uninfected children. This aligns with a lower incidence of caries in primary teeth at age 6 or less for exposed yet uninfected children. Ultimately, these findings could contribute to improved risk assessment, intervention, and prevention strategies such as biofilm disruption and the informed design of pro-, pre-, and synbiotic oral therapies.
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影响因子：
20.3
作者：
Clerici, M;Saresella, M;Vigano, A
通讯作者：
Vigano, A
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
5.5
作者：
Abdelmoez, Ahmed M.;Puig, Laura Sardon;Pillon, Nicolas J.
通讯作者：
Pillon, Nicolas J.
影响因子：
3.6
作者：
Coker, Modupe;El-Kamary, Samer S.;Charurat, Manhattan
通讯作者：
Charurat, Manhattan
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y