Delivery of water-soluble drugs using acoustically triggered perfluorocarbon double emulsions.
Delivery of water-soluble drugs using acoustically triggered perfluorocarbon double emulsions.
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DOI：
10.1007/s11095-010-0277-5
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发表时间：
2010-12
影响因子：
3.7
通讯作者：
Fowlkes, J. Brian
中科院分区：
文献类型：
--
作者：
Fabiilli, Mario L.;Lee, James A.;Kripfgans, Oliver D.;Carson, Paul L.;Fowlkes, J. Brian
关键词：
超声波可用于通过声学液滴汽化(ADV)释放包裹在全氟化碳(PFC)乳剂中的治疗有效载荷，即PFC相被蒸发并释放试剂的过程。ADV产生的微泡以前被用来选择性地闭塞体内的血管。ADV产生的药物传递和闭塞的耦合具有治疗、协同作用的潜力。以全氟戊烷(PFP)或全氟己烷(PFH)为PFC相，采用两步法制备了微米级水包水(W1/PFC/W2)乳状液。W1相中含有荧光素或凝血酶。用Franz扩散池测量，在W1相含荧光素的双乳剂在ADV后，PFP和PFH乳剂的荧光素质量通量分别增加了5.7±1.4倍和8.2±1.3倍。凝血酶在五分之四的双乳剂中稳定保留。对于所测试的五种配方中的三种，与未暴露在超声波下的乳剂相比，在超声作用下与装载凝血酶的乳剂孵育时，全血凝血时间缩短了，具有统计学意义(p<；0.01)。ADV可以用来在空间和时间上控制PFC双乳剂中配制的水溶性化合物的输送。凝血酶的释放可以延长ADV引起的微泡闭塞的持续时间。
Ultrasound can be used to release a therapeutic payload encapsulated within a perfluorocarbon (PFC) emulsion via acoustic droplet vaporization (ADV), a process whereby the PFC phase is vaporized and the agent is released. ADV-generated microbubbles have been previously used to selectively occlude blood vessels in vivo. The coupling of ADV-generated drug delivery and occlusion has therapeutically, synergistic potentials. Micron-sized, water-in-PFC-in-water (W1/PFC/W2) emulsions were prepared in a two-step process using perfluoropentane (PFP) or perfluorohexane (PFH) as the PFC phase. Fluorescein or thrombin was contained in the W1 phase. Double emulsions containing fluorescein in the W1 phase displayed a 5.7±1.4 fold and 8.2±1.3 fold increase in fluorescein mass flux, as measured using a Franz diffusion cell, after ADV for the PFP and PFH emulsions, respectively. Thrombin was stably retained in four out of five double emulsions. For three out of five formulations tested, the clotting time of whole blood decreased, in a statistically significant manner (p < 0.01), when incubated with thrombin-loaded emulsions exposed to ultrasound compared to emulsions not exposed to ultrasound. ADV can be used to spatially and temporally control the delivery of water-soluble compounds formulated in PFC double emulsions. Thrombin release could extend the duration of ADV-generated, microbubble occlusions.
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DOI：
10.1016/j.colsurfa.2004.06.003
发表时间：
2004-09-06
影响因子：
5.2
作者：
Courrier, HA;Vandamme, TF;Krafft, MP
通讯作者：
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影响因子：
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作者：
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DOI：
10.1109/tuffc.2009.1132
发表时间：
2009-05
期刊：
IEEE transactions on ultrasonics, ferroelectrics, and frequency control
影响因子：
--
作者：
Fabiilli ML;Haworth KJ;Fakhri NH;Kripfgans OD;Carson PL;Fowlkes JB
通讯作者：
Fowlkes JB
影响因子：
4.8
作者：
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通讯作者：
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影响因子：
2.9
作者：
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通讯作者：
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