AtHMA4 Drives Natural Variation in Leaf Zn Concentration of Arabidopsis thaliana.
AtHMA4 Drives Natural Variation in Leaf Zn Concentration of Arabidopsis thaliana.
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DOI：
10.3389/fpls.2018.00270
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发表时间：
2018
影响因子：
5.6
通讯作者：
Chao DY
中科院分区：
文献类型：
--
作者：
Chen ZR;Kuang L;Gao YQ;Wang YL;Salt DE;Chao DY
锌（Zn）是植物生长发育的必需元素，膳食中来自农作物的锌对人体健康也很重要。在这里，我们报告，重金属-ATP酶4（HMA 4）的遗传变异控制叶片锌含量的自然变化。在全世界收集的349个拟南芥野生收集的种质中，对叶Zn含量的自然变化的研究鉴定了两个种质，货车-0和Fab-2，当与Col-0相比时，其积累显著较低的Zn。数量性状基因座（QTL）分析和混合分离分析（BSA）确定HMA 4作为一个强有力的候选人占这种变化的叶片锌浓度。遗传互补实验证实了这一假设。序列分析显示，Fab-2中HMA 4第三外显子中的1-bp缺失导致HMA 4功能丧失，从而导致Fab-2中观察到的低Zn。与Fab-2不同，发现启动子区的多态性是货车-0中HMA 4功能弱的原因。这得到了货车-0中HMA 4的表达分析和一系列在Col-0背景下产生截短的HMA 4启动子的T-DNA插入突变体的支持。此外，我们还观察到Fab-2，货车-0和hma 4 -2无效突变体在Col-0背景下显示出增强的抗性组合的高锌和高镉的生长培养基中，提高的可能性，在HMA 4的变化可能在环境适应中发挥作用。
Zinc (Zn) is an essential element for plant growth and development, and Zn derived from crop plants in the diet is also important for human health. Here, we report that genetic variation in Heavy Metal-ATPase 4 (HMA4) controls natural variation in leaf Zn content. Investigation of the natural variation in leaf Zn content in a world-wide collection of 349 Arabidopsis thaliana wild collected accessions identified two accessions, Van-0 and Fab-2, which accumulate significantly lower Zn when compared with Col-0. Both quantitative trait loci (QTL) analysis and bulked segregant analysis (BSA) identified HMA4 as a strong candidate accounting for this variation in leaf Zn concentration. Genetic complementation experiments confirmed this hypothesis. Sequence analysis revealed that a 1-bp deletion in the third exon of HMA4 from Fab-2 is responsible for the lose of function of HMA4 driving the low Zn observed in Fab-2. Unlike in Fab-2 polymorphisms in the promoter region were found to be responsible for the weak function of HMA4 in Van-0. This is supported by both an expression analysis of HMA4 in Van-0 and through a series of T-DNA insertion mutants which generate truncated HMA4 promoters in the Col-0 background. In addition, we also observed that Fab-2, Van-0 and the hma4-2 null mutant in the Col-0 background show enhanced resistance to a combination of high Zn and high Cd in the growth medium, raising the possibility that variation at HMA4 may play a role in environmental adaptation.
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影响因子：
4.5
作者：
Baxter I;Brazelton JN;Yu D;Huang YS;Lahner B;Yakubova E;Li Y;Bergelson J;Borevitz JO;Nordborg M;Vitek O;Salt DE
通讯作者：
Salt DE
影响因子：
3.3
作者：
Kronholm, Ilkka;Xavier Pico, F.;de Meaux, Juliette
通讯作者：
de Meaux, Juliette
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
DOI：
10.1073/pnas.95.12.7220
发表时间：
1998-06-09
影响因子：
11.1
作者：
Grotz, N;Fox, T;Eide, D
通讯作者：
Eide, D
影响因子：
4.5
作者：
Campbell MT;Bandillo N;Al Shiblawi FRA;Sharma S;Liu K;Du Q;Schmitz AJ;Zhang C;Véry AA;Lorenz AJ;Walia H
通讯作者：
Walia H