Comparison of electron spin resonance spectroscopy and inductively-coupled plasma optical emission spectroscopy for biodistribution analysis of iron-oxide nanoparticles.
Comparison of electron spin resonance spectroscopy and inductively-coupled plasma optical emission spectroscopy for biodistribution analysis of iron-oxide nanoparticles.
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DOI：
10.1021/mp900161h
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发表时间：
2010-04-05
影响因子：
4.9
通讯作者：
Yang VC
中科院分区：
文献类型：
--
作者：
Chertok B;Cole AJ;David AE;Yang VC
磁性纳米颗粒(MNP)在靶向给药方面得到了广泛的研究。分析MNP的生物分布对于评估靶向策略的成功和靶外毒性的可能性至关重要。本研究比较了电感耦合等离子体光发射光谱(ICP-OES)和电子自旋共振(ESR)光谱在评价MNP生物分布中的适用性。在磁靶向下观察MNP(12~25 mg Fe/kg)在荷9L胶质瘤大鼠体内的生物分布。用电感耦合等离子体发射光谱仪和电子自旋共振对动物组织中的MNP进行体外分析。一种低温方法被开发出来，以克服将组织样本加载到ESR管中的技术障碍。对电感耦合等离子体发射光谱和血沉测量结果的比较表明，器官积累高或低水平的MNP有两种截然不同的关系。在肝和脾等高MNP积累的器官中，数据具有很强的相关性(肝和脾的相关系数分别为0.97和0.94)，从而验证了这两种方法在这个高浓度范围内的等效性(>；1000nmolFe/g组织)。然而，这两组测量结果在大脑、肾脏和肿瘤等MNP积累水平较低的器官中存在显著差异。ESR可将MNP分解到10-55nmolFe/g组织，而由于内源性铁的掩蔽，电感耦合等离子体发射光谱仪未能检测到MNP。这些发现表明，与低温样品处理相结合的电子自旋共振比电感耦合等离子体发射光谱更可靠，在分析中获得了更好的灵敏度。这些优点使ESR成为准确分析纳米颗粒累积变异性高的组织中MNP生物分布的选择方法。
Magnetic nanoparticles (MNP) have been widely studied for use in targeted drug delivery. Analysis of MNP biodistribution is essential to evaluating the success of targeting strategies and the potential for off-target toxicity. This work compared the applicability of inductively-coupled plasma optical emission spectroscopy (ICP-OES) and electron spin resonance (ESR) spectroscopy in assessing MNP biodistribution. Biodistribution was evaluated in 9L-glioma bearing rats administered with MNP (12-25 mg Fe/kg) under magnetic targeting. Ex vivo analysis of MNP in animal tissues was performed with both ICP-OES and ESR. A cryogenic method was developed to overcome the technical hurdle of loading tissue samples into ESR tubes. Comparison of results from the ICP-OES and ESR measurements revealed two distinct relationships for organs accumulating high or low levels of MNP. In organs with high MNP accumulation such as liver and spleen, data were strongly correlated (r = 0.97, 0.94 for liver and spleen, respectively), thus validating equivalency of the two methods in this high concentration range (> 1000 nmol Fe/g tissue). The two sets of measurements, however, differed significantly in organs with lower levels of MNP accumulation such as brain, kidney, and the tumor. Whereas ESR resolved MNP to 10-55 nmol Fe/g tissue, ICP-OES failed to detect MNP due to masking by endogenous iron. These findings suggest that ESR coupled to cryogenic sample handling is more robust than ICP-OES, attaining better sensitivity in analyses. Such advantages render ESR the method of choice for accurate profiling of MNP biodistribution across tissues with high variability in nanoparticle accumulation.
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影响因子：
5
作者：
MARCHAL, G;VANHECKE, P;VANDERSCHUEREN, E
通讯作者：
VANDERSCHUEREN, E
影响因子：
3.9
作者：
Jordan, A;Scholz, R;Felix, R
通讯作者：
Felix, R
DOI：
10.1016/j.bbrc.2008.06.126
发表时间：
2008-09-19
影响因子：
3.1
作者：
Kou, Geng;Wang, Shuhui;Guo, Yajun
通讯作者：
Guo, Yajun
影响因子：
2.7
作者：
Lazaro, Francisco Jose;Gutierrez, Lucia;Munoz, Maria Jesus
通讯作者：
Munoz, Maria Jesus
影响因子：
2.7
作者：
Mykhaylyk, O;Dudchenko, N;Dudchenko, A
通讯作者：
Dudchenko, A