circRNA hsa_circ_0005909 Predicts Poor Prognosis and Promotes the Growth, Metastasis, and Drug Resistance of Non-Small-Cell Lung Cancer via the miRNA-338-3p/SOX4 Pathway.
circRNA hsa_circ_0005909 Predicts Poor Prognosis and Promotes the Growth, Metastasis, and Drug Resistance of Non-Small-Cell Lung Cancer via the miRNA-338-3p/SOX4 Pathway.
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DOI：
10.1155/2021/8388512
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Zhang XY
中科院分区：
文献类型：
--
作者：
Song HM;Meng D;Wang JP;Zhang XY
环状RNAs(CircRNAs)是调节多种癌症行为的强大因子。已有研究表明，环状RNA hsa_circ_0005909(Circ_0005909)在骨肉瘤中具有调节作用。然而，目前还没有关于CIRC_0005909在非小细胞肺癌进展中是否发挥潜在作用的其他研究。逆转录聚合酶链式反应检测CIRC_0005909在非小细胞肺癌中的表达。采用细胞计数试剂盒8(CCK-8)法、集落形成实验、Transwell实验和裸鼠移植瘤模型实验等方法，研究了C1RC_0005909基因敲除后NSCLC细胞的特异性行为。应用生物信息学和荧光素酶报告基因分析方法研究了CIRC_0005909、miRNA338-3p和Sox4之间的关系。在这项研究中，本课题组首次发现CIRC_0005909在非小细胞肺癌标本和细胞系中的表达明显增加。临床研究表明，CIRC_0005909高表达与非小细胞肺癌患者预后不良有关。在功能上，CIRC_0005909基因的敲除可以抑制非小细胞肺癌细胞的增殖、转移和耐药。在机制上，CIRC_0005909可以作为miRNA-338-3p的海绵，miRNA-338-3p可以靶向Sox4。此外，miRNA338-3p抑制剂还逆转了CIRC_0005909沉默对非小细胞肺癌行为的抑制作用。CIRC_0005909通过调节miRNA338-3p/Sox4轴促进非小细胞肺癌的进展，可能成为非小细胞肺癌的治疗靶点。
Circular RNAs (circRNAs) are powerful factors in regulating various cancer behaviors. It has been manifested in previous researches that circular RNA hsa_circ_0005909 (circ_0005909) exhibits a regulatory function in osteosarcoma. However, there are no other studies on whether circ_0005909 displays potential functions on the progression of non-small-cell lung cancer (NSCLC). RT-PCR was applied to examine the expression of circ_0005909 in NSCLC. To study the specific behaviors of NSCLC cells after circ_0005909 knockdown, cell counting kit-8 (CCK-8) assays, colony formation assays, Transwell assays, and xenograft tumor model assays were conducted. Bioinformatics and luciferase reporter assays were employed to study the association among circ_0005909, miRNA-338-3p, and SOX4. In this research, our group firstly showed that circ_0005909 expressions were distinctly increased in NSCLC specimens and cell lines. Clinical studies revealed that high circ_0005909 expressions were associated with poor prognosis of NSCLC patients. Functionally, knockdown of circ_0005909 was observed to suppress the proliferation, metastasis, and drug resistance of NSCLC cells. In the terms of mechanism, circ_0005909 could act as a sponge of miRNA-338-3p, and miRNA-338-3p could target SOX4. In addition, miRNA-338-3p inhibitors reversed the suppressor ability of circ_0005909 silence on NSCLC behaviors. circ_0005909 promoted the progression of NSCLC via the modulation of the miRNA-338-3p/SOX4 axis, which may be a therapeutic target for NSCLC.
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影响因子：
9
作者：
Li H;Liu Q;Chen Z;Wu M;Zhang C;Su J;Li Y;Zhang C
通讯作者：
Zhang C
影响因子：
28.2
作者：
Karreth FA;Pandolfi PP
通讯作者：
Pandolfi PP
circRNA生物合成的调控
影响因子：
4.1
作者：
Chen, Ling-Ling;Yang, Li
通讯作者：
Yang, Li
DOI：
10.1007/978-981-13-1426-1_6
发表时间：
2018-01-01
期刊：
CIRCULAR RNAS: BIOGENESIS AND FUNCTIONS
影响因子：
--
作者：
Panda, Amaresh Chandra
通讯作者：
Panda, Amaresh Chandra
影响因子：
3
作者：
Duan, Zhihui;Wei, Shuqing;Liu, Ying
通讯作者：
Liu, Ying