Genome sequencing of Giardia lamblia genotypes A2 and B isolates (DH and GS) and comparative analysis with the genomes of genotypes A1 and E (WB and Pig).
Genome sequencing of Giardia lamblia genotypes A2 and B isolates (DH and GS) and comparative analysis with the genomes of genotypes A1 and E (WB and Pig).
复制标题
DOI：
10.1093/gbe/evt197
复制
发表时间：
2013
影响因子：
3.3
通讯作者：
Nash TE
中科院分区：
文献类型：
--
作者：
Adam RD;Dahlstrom EW;Martens CA;Bruno DP;Barbian KD;Ricklefs SM;Hernandez MM;Narla NP;Patel RB;Porcella SF;Nash TE
蓝氏贾第鞭毛虫（Giardia lamblia，syn G. Pasta，G.肠寄生虫病是世界范围内人类最常见的致病性肠道寄生虫，是地方性和流行性腹泻的常见原因。G.兰氏杆菌分为八种基因型（A-H），其感染广泛的哺乳动物和人类，但人类感染由基因型A和B引起。为了明确确定基因型之间的关系，我们对GS和DH（基因型B和A2）进行了高深度覆盖测序，并将组装体与基因型E（P15;猪分离株）和B（GS;人分离株）的几乎完整的WB基因组和测序调查草案进行了比较。我们的结果确定DH是迄今为止测序的最小的贾第虫基因组，而GS是最大的。开放阅读框架分析和系统发育分析表明，GS与其他三个基因组的距离比其他三个基因组之间的距离要远。DH_A2和GS_B与光学作图的WB_A1的全基因组比较表明，在所有五条染色体上都存在大量的同线性，但也包括一些重排、倒位和染色体易位，这些重排、倒位和易位在染色体末端更常见。然而，WB_A1/GS_B比对表明，在同线区域中只有约70%的序列同一性。我们的研究结果增加了以前的报告中提出的信息，表明GS是一个不同的贾第鞭毛虫物种的基因组多样性的程度，编码能力，杂合性，系统发育距离，和已知的生物学差异WB_A1和其他G。Lamblia基因型。
Giardia lamblia (syn G. intestinalis, G. duodenalis) is the most common pathogenic intestinal parasite of humans worldwide and is a frequent cause of endemic and epidemic diarrhea. G. lamblia is divided into eight genotypes (A–H) which infect a wide range of mammals and humans, but human infections are caused by Genotypes A and B. To unambiguously determine the relationship among genotypes, we sequenced GS and DH (Genotypes B and A2) to high depth coverage and compared the assemblies with the nearly completed WB genome and draft sequencing surveys of Genotypes E (P15; pig isolate) and B (GS; human isolate). Our results identified DH as the smallest Giardia genome sequenced to date, while GS is the largest. Our open reading frame analyses and phylogenetic analyses showed that GS was more distant from the other three genomes than any of the other three were from each other. Whole-genome comparisons of DH_A2 and GS_B with the optically mapped WB_A1 demonstrated substantial synteny across all five chromosomes but also included a number of rearrangements, inversions, and chromosomal translocations that were more common toward the chromosome ends. However, the WB_A1/GS_B alignment demonstrated only about 70% sequence identity across the syntenic regions. Our findings add to information presented in previous reports suggesting that GS is a different species of Giardia as supported by the degree of genomic diversity, coding capacity, heterozygosity, phylogenetic distance, and known biological differences from WB_A1 and other G. lamblia genotypes.
登录
查看更多内容
DOI：
10.1073/pnas.221575398
发表时间：
2002-01-08
影响因子：
11.1
作者：
DelVecchio, VG;Kapatral, V;Overbeek, R
通讯作者：
Overbeek, R
影响因子：
9.2
作者：
Cooper, Margarethe A.;Adam, Rodney D.;Sterling, Charles R.
通讯作者：
Sterling, Charles R.
影响因子：
--
作者：
Beare, Paul A.;Chen, Chen;Heinzen, Robert A.
通讯作者：
Heinzen, Robert A.
影响因子：
6.4
作者：
Haque, R;Roy, S;Houpt, ER
通讯作者：
Houpt, ER
影响因子：
168.9
作者：
GILMAN, RH;MIRANDA, E;MARTINEZ, H
通讯作者：
MARTINEZ, H