Gene expression divergence between malaria vector sibling species Anopheles gambiae and An. coluzzii from rural and urban Yaoundé Cameroon.
Gene expression divergence between malaria vector sibling species Anopheles gambiae and An. coluzzii from rural and urban Yaoundé Cameroon.
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DOI：
10.1111/mec.12733
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发表时间：
2014-05
影响因子：
4.9
通讯作者：
Besansky NJ
中科院分区：
文献类型：
--
作者：
Cassone BJ;Kamdem C;Cheng C;Tan JC;Hahn MW;Costantini C;Besansky NJ
水生幼虫生态学的基础上的分歧选择是一个可能的因素，在最近隔离的两个广泛同域和形态相同的非洲蚊子物种，疟疾媒介冈比亚按蚊和安。猫猴。基于种群的基因组扫描揭示了许多最近正选择的候选区域，但对两个物种之间行为和生理差异的遗传机制提供的线索很少，表型本身仍然不清楚。为了揭示可能的遗传机制，我们使用基于基因的微阵列比较了自然群体和实验群体的全球转录谱。在喀麦隆首都雅温得市及其周围的自然种群中，两个物种沿着城市化的梯度分离，作为第二和第四龄幼虫进行采样。差异表达基因的功能富集分析显示，An. coluzzii-在更稳定，生物复杂和潜在污染的城市水体中繁殖的物种-相对于An过度表达与解毒和免疫有关的基因。冈比亚，它在郊区和农村地区的较短暂和相对落后的池塘和水坑中繁殖。此外，我们的数据表明，这种过度表达的安。coluzzii不是一个短暂的结果，诱导外源性物质在幼虫的栖息地，但一个固有的和可能的适应性反应，反复遇到的环境压力。最后，我们发现差异表达的基因座和以前确定的基因组区域最近的积极选择之间没有显着的重叠，这表明转录组分歧是由反式作用因子，而不是顺式作用元件。
Divergent selection based on aquatic larval ecology is a likely factor in the recent isolation of two broadly sympatric and morphologically identical African mosquito species, the malaria vectors Anopheles gambiae and An. coluzzii. Population-based genome scans have revealed numerous candidate regions of recent positive selection, but have provided few clues as to the genetic mechanisms underlying behavioral and physiological divergence between the two species, phenotypes which themselves remain obscure. To uncover possible genetic mechanisms, we compared global transcriptional profiles of natural and experimental populations using gene-based microarrays. Larvae were sampled as second and fourth instars from natural populations in and around the city of Yaoundé, capital of Cameroon, where the two species segregate along a gradient of urbanization. Functional enrichment analysis of differentially expressed genes revealed that An. coluzzii—the species that breeds in more stable, biotically complex and potentially polluted urban water bodies—over-expresses genes implicated in detoxification and immunity relative to An. gambiae, which breeds in more ephemeral and relatively depauperate pools and puddles in suburbs and rural areas. Moreover, our data suggest that such over-expression by An. coluzzii is not a transient result of induction by xenobiotics in the larval habitat, but an inherent and presumably adaptive response to repeatedly encountered environmental stressors. Finally, we find no significant overlap between the differentially expressed loci and previously identified genomic regions of recent positive selection, suggesting that transcriptome divergence is regulated by trans-acting factors rather than cis-acting elements.
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DOI：
10.1126/science.1193162
发表时间：
2010-10-01
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Bartholomay LC;Waterhouse RM;Mayhew GF;Campbell CL;Michel K;Zou Z;Ramirez JL;Das S;Alvarez K;Arensburger P;Bryant B;Chapman SB;Dong Y;Erickson SM;Karunaratne SH;Kokoza V;Kodira CD;Pignatelli P;Shin SW;Vanlandingham DL;Atkinson PW;Birren B;Christophides GK;Clem RJ;Hemingway J;Higgs S;Megy K;Ranson H;Zdobnov EM;Raikhel AS;Christensen BM;Dimopoulos G;Muskavitch MA
通讯作者：
Muskavitch MA
影响因子：
6.7
作者：
Dong Y;Aguilar R;Xi Z;Warr E;Mongin E;Dimopoulos G
通讯作者：
Dimopoulos G
影响因子：
4.9
作者：
Cassone, Bryan J.;Molloy, Matthew J.;Besansky, Nora J.
通讯作者：
Besansky, Nora J.
影响因子：
4.4
作者：
David, Jean-Philippe;Coissac, Eric;Reynaud, Stephane
通讯作者：
Reynaud, Stephane
影响因子：
3.8
作者：
della Torre, A;Tu, ZJ;Petrarca, V
通讯作者：
Petrarca, V