Evaluation of Macaca radiata as a non-human primate model of Dengue virus infection.
Evaluation of Macaca radiata as a non-human primate model of Dengue virus infection.
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DOI：
10.1038/s41598-018-21582-9
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发表时间：
2018-02-21
影响因子：
4.6
通讯作者：
Hishiki T
中科院分区：
文献类型：
--
作者：
Kato F;Ishida Y;Kawakami A;Takasaki T;Saijo M;Miura T;Hishiki T
登革热病毒（DENV）在人类中引起多种疾病，包括登革热和登革出血热。目前DENV感染的动物模型在理解人类传染病方面是有限的。帽猴（Macaca radiata）是一种旧大陆猴，已被用于研究黄病毒的实验和自然感染，但旧大陆猴尚未被用作DENV感染模型。在这项研究中，几种DENV菌株的复制水平评估使用外周血单核细胞。我们的研究结果表明，从2009年从印度返回的旅行者身上分离出的DENV-4 09-48毒株是一种高度复制的病毒。用3只帽猴感染09-48株，观察抗体反应。检测DENV非结构蛋白1抗原，观察到高病毒血症。这些结果表明，帽猴和09-48株可以作为研究DENV的可靠灵长类动物模型。
Dengue virus (DENV) causes a wide range of illnesses in humans, including dengue fever and dengue haemorrhagic fever. Current animal models of DENV infection are limited for understanding infectious diseases in humans. Bonnet monkeys (Macaca radiata), a type of Old World monkey, have been used to study experimental and natural infections by flaviviruses, but Old World monkeys have not yet been used as DENV infection models. In this study, the replication levels of several DENV strains were evaluated using peripheral blood mononuclear cells. Our findings indicated that DENV-4 09-48 strain, isolated from a traveller returning from India in 2009, was a highly replicative virus. Three bonnet monkeys were infected with 09-48 strain and antibody responses were assessed. DENV nonstructural protein 1 antigen was detected and high viraemia was observed. These results indicated that bonnet monkeys and 09-48 strain could be used as a reliable primate model for the study of DENV.
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