Dengue virus serotype 2 from a sylvatic lineage isolated from a patient with dengue hemorrhagic fever.
Dengue virus serotype 2 from a sylvatic lineage isolated from a patient with dengue hemorrhagic fever.
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DOI：
10.1371/journal.pntd.0000423
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发表时间：
2009
影响因子：
3.8
通讯作者：
Abdul Manap Z
中科院分区：
文献类型：
--
作者：
Cardosa J;Ooi MH;Tio PH;Perera D;Holmes EC;Bibi K;Abdul Manap Z
登革病毒在人类和森林循环中传播。尽管登革病毒（DENV）感染人类可引起重大流行和严重疾病，但对森林登革病毒的流行病学和病原学知之甚少。2008年1月，一名20岁的男性在马来西亚出现登革出血热（DHF）伴血小板减少（12，000/ul）和红细胞压积升高（高于基线29.5%）。在发烧第4天，从他的血液中分离出登革热病毒血清2型。完整基因组序列的系统发育分析显示，该病毒是DENV-2的森林谱系的成员，与1970年从马来西亚哨兵猴中分离的病毒最密切相关。这是自1975年以来首次鉴定出在亚洲流行的森林型DENV。登革病毒是蚊媒RNA病毒，可引起从轻微疾病到危及生命的登革出血热（DHF）的一系列疾病。登革病毒在自然界中存在两个独立的循环，在非人类灵长类动物或人类中传播。今天在人类中流行的病毒很可能是从几百年前的非人灵长类登革热病毒进化而来的，此后在人群中形成了四种不同的血清型，导致周期性流行病和严重疾病。在2008年马来西亚登革热传播的常规监测过程中，从一名患有登革出血热的年轻男性中分离出了一种2型森林登革热病毒。该病毒的完整基因组序列被确定，并发现与1970年从马来西亚类似地区的猴子中分离出的病毒密切相关。这是30多年来在亚洲分离出的第一个森林登革热病毒。
Dengue viruses circulate in both human and sylvatic cycles. Although dengue viruses (DENV) infecting humans can cause major epidemics and severe disease, relatively little is known about the epidemiology and etiology of sylvatic dengue viruses. A 20-year-old male developed dengue hemorrhagic fever (DHF) with thrombocytopenia (12,000/ul) and a raised hematocrit (29.5% above baseline) in January 2008 in Malaysia. Dengue virus serotype 2 was isolated from his blood on day 4 of fever. A phylogenetic analysis of the complete genome sequence revealed that this virus was a member of a sylvatic lineage of DENV-2 and most closely related to a virus isolated from a sentinel monkey in Malaysia in 1970. This is the first identification of a sylvatic DENV circulating in Asia since 1975. Dengue viruses are mosquito-borne RNA viruses that cause a spectrum of illness from mild disease to life-threatening dengue hemorrhagic fever (DHF). Dengue viruses exist in two separate cycles in nature, circulating in either non-human primates or humans. The viruses that are endemic in humans today most likely evolved from non-human primate dengue viruses a few hundred years ago and have since established themselves as four distinct serotypes in human populations, causing periodic epidemics and severe disease. During the course of routine surveillance for dengue transmission in Malaysia during 2008, a sylvatic dengue virus type 2 was isolated from a young human male with DHF. The complete genome sequence of the virus was determined and found to be closely related to that isolated from a monkey in a similar region of Malaysia in 1970. This is the first sylvatic dengue virus isolated in Asia for more than 30 years.
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影响因子：
3.7
作者：
Vasilakis, Nikos;Shell, Elisabeth J.;Weaver, Scott C.
通讯作者：
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森林登革热病毒 2 型在人类中的活动，尼日利亚，1966 年。
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作者：
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影响因子：
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通讯作者：
Chang, GJJ
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作者：
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通讯作者：
Guzman, Maria G.
影响因子：
5.4
作者：
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