Non-cytotoxic Thymus capitata extracts prevent Bovine herpesvirus-1 infection in cell cultures.
Non-cytotoxic Thymus capitata extracts prevent Bovine herpesvirus-1 infection in cell cultures.
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DOI：
10.1186/s12917-014-0231-6
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发表时间：
2014-09-27
影响因子：
2.6
通讯作者：
Ghram A
中科院分区：
文献类型：
--
作者：
Boubaker-Elandalousi R;Mekni-Toujani M;Kaabi B;Larbi I;Diouani MF;Gharbi M;Akkari H;B'chir F;Ghram A
牛疱疹病毒1型（BHV-1）的感染给畜牧业和养殖业造成了巨大的经济损失，目前尚无有效的抗BHV-1的药物，本研究旨在评价红头草提取物的细胞毒性和抗病毒活性，为开发新型、无毒、特异性的抗疱疹病毒药物奠定基础。采用气相色谱、高效液相色谱-质谱联用技术，分析了头状百里香地上部分水提物（AE）、醇提物（EE）和挥发油（EO）的化学成分。采用Madin-Darby牛肾细胞系，采用比色法测定其对牛疱疹病毒1型（BHV-1）的细胞毒性和抗病毒活性。T.在病毒感染的不同阶段加入capitata提取物以研究和更好地量化它们潜在的抑制作用。多酚类和黄酮类化合物是T. Capitata EO、EE和AE。EO、EE和AE的50%细胞毒性浓度分别为48.70、189和289 μg ml−1。EO、EE和AE在50%时的抑制剂浓度分别为3.36、47.80和164 μg ml-1。EO、EE和AE的选择性指数抗BHV-1值分别为14.49、3.95和1.81。因此，EO浸提物是最有效的抗病毒化合物。T. capitata提取物主要影响BHV-1病毒对宿主细胞的吸附。T. capitata提取物通过干扰病毒吸附和复制的早期阶段来抑制病毒复制。因此，T. capitata是抗疱疹病毒治疗的潜在候选物。
Bovine herpes virus type 1 (BHV-1) still causes great economic loss to the livestock industry and trade because there aren’t any available drugs that proved to be fully effective against it. In this study, the cytotoxicity and the antiviral activities of the Thymus capitata extracts were evaluated for the development of new, non toxic and specific anti-herpesvirus drug. Aqueous extracts (AE), ethanolic extracts (EE) and essential oil (EO) of the aerial parts of Thymus capitata were analyzed to determine their chemical compositions by gas chromatography, and high performance liquid chromatography combined with mass spectrometry. Their cytotoxicity and antiviral activities against Bovine Herpesvirus type 1 (BHV-1) were evaluated by quantifying the reduction of the viral cytopathic effect using Madin-Darby Bovine Kidney cell line with colorimetric assay. T. capitata extracts were added at different stages of the viral infection to investigate and better quantify their potential inhibitory effects. Polyphenols and flavonoids were the major compounds found in T. capitata EO, EE and AE. The cytotoxic concentrations at 50% were 48.70, 189 and 289 μg ml−1 for EO, EE and AE, respectively. The inhibitor concentrations at 50% for the EO, EE and AE, were 3.36, 47.80 and 164 μg ml−1, respectively. The selectivity index anti-BHV-1 values were 14.49, 3.95 and 1.81 for EO, EE and AE, respectively. Thus, the EO extracts were the most efficient antiviral compounds. T. capitata extracts affect mainly the adsorption of BHV-1 virus to host cells. T. capitata extracts inhibit the viral replication by interfering with the early stages of viral adsorption and replication. Thus, T. capitata is a potential candidate for anti-herpesvirus treatment.
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影响因子：
5.4
作者：
Chattopadhyay D;Sarkar MC;Chatterjee T;Sharma Dey R;Bag P;Chakraborti S;Khan MT
通讯作者：
Khan MT
影响因子：
2
作者：
Mestrimer Felipe, Adriana Meri;Rincao, Viniclus Pires;Nozawa, Carlos
通讯作者：
Nozawa, Carlos
影响因子：
3.8
作者：
Ozcelik, Berrin;Kartal, Murat;Orhan, Ilkay
通讯作者：
Orhan, Ilkay
影响因子：
5.6
作者：
Jabri-Karoui, Iness;Bettaieb, Iness;Marzouk, Brahim
通讯作者：
Marzouk, Brahim
影响因子：
1.6
作者：
Loziene, K;Venskutonis, PR
通讯作者：
Venskutonis, PR