Electrical synaptic transmission in developing zebrafish: properties and molecular composition of gap junctions at a central auditory synapse.
Electrical synaptic transmission in developing zebrafish: properties and molecular composition of gap junctions at a central auditory synapse.
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发育中的斑马鱼的电突触传递：中央听觉突触间隙连接的特性和分子组成。
DOI：
10.1152/jn.00397.2014
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发表时间：
2014
影响因子：
2.5
通讯作者：
Pereda,AlbertoE
中科院分区：
文献类型：
--
作者：
Yao,Cong;Vanderpool,KimberlyG;Delfiner,Matthew;Eddy,Vanessa;Lucaci,AlexanderG;Soto-Riveros,Carolina;Yasumura,Thomas;Rash,JohnE;Pereda,AlbertoE
与化学突触的知识相反，关于发育中的斑马鱼中间隙连接介导的电突触的性质知之甚少，这为在系统水平上研究神经功能提供了有价值的模型。Mauthner细胞（2个参与甩尾逃避反应的大型网状脊髓神经元）上可识别的“混合”（电和化学）听觉突触接触被称为“俱乐部末梢”，允许探索鱼类的电传输。在这里，我们表明，平行的听觉反应的发展，在这些接触的电突触成为解剖学上可识别的受精后第3天，达到了1006之间的第4和第9天。此外，每个末端含有1018个间隙连接，代表由哺乳动物连接蛋白36的硬骨鱼同源物形成的2，000至3，000个连接子通道。电生理记录显示，在每个这些接触的间隙连接是功能性的，突触传递具有与成鱼相媲美的特性。因此，令人惊讶的是，少数混合突触负责Mauthner细胞获得听觉反应，这些突触可能足以支持早期发育阶段的逃避行为。
In contrast to the knowledge of chemical synapses, little is known regarding the properties of gap junction-mediated electrical synapses in developing zebrafish, which provide a valuable model to study neural function at the systems level. Identifiable “mixed” (electrical and chemical) auditory synaptic contacts known as “club endings” on Mauthner cells (2 large reticulospinal neurons involved in tail-flip escape responses) allow exploration of electrical transmission in fish. Here, we show that paralleling the development of auditory responses, electrical synapses at these contacts become anatomically identifiable atday 3postfertilization, reaching a number of ∼6 betweendays 4and9. Furthermore, each terminal contains ∼18 gap junctions, representing between 2,000 and 3,000 connexon channels formed by the teleost homologs of mammalian connexin 36. Electrophysiological recordings revealed that gap junctions at each of these contacts are functional and that synaptic transmission has properties that are comparable with those of adult fish. Thus a surprisingly small number of mixed synapses are responsible for the acquisition of auditory responses by the Mauthner cells, and these are likely sufficient to support escape behaviors at early developmental stages.
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