Electrochemical Measurements Reveal Reactive Oxygen Species in Stress Granules*.
Electrochemical Measurements Reveal Reactive Oxygen Species in Stress Granules*.
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DOI：
10.1002/anie.202104308
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发表时间：
2021-07-05
期刊：
影响因子：
--
通讯作者：
Ewing AG
中科院分区：
文献类型：
--
作者：
Hu K;Relton E;Locker N;Phan NTN;Ewing AG
应激颗粒（SG）是无膜细胞器，在细胞质中组装以组织细胞内容物并促进应激期间的快速适应。为了了解SG如何有助于生理功能，我们使用电化学测量来检测SG中的电活性物质。通过电流分析，我们发现活性氧（ROS）被封装在亚砷酸盐诱导的SGs中，H2O2是主要物种。定量分析了单根SGs释放H2O2的动力学和H2O2分子的数量。SGs含有ROS的发现暗示它们是细胞应激的通讯者，而不是简单的终点。这可能解释了SGs如何调节细胞代谢和应激反应。这也可能有助于更好地了解它们在与SG相关的病理条件下的细胞保护功能，例如神经退行性疾病（ND），癌症和病毒感染。从非癌细胞和癌细胞中分离的亚砷酸盐诱导的应激颗粒含有活性氧，如使用铂或铂化碳微电极的安培测量所示。这一发现支持SG作为信号中枢的作用，并揭示了SG生物学与神经退行性疾病和某些癌症发病机制之间的相关性。
Stress granules (SGs) are membrane‐less organelles that assemble in the cytoplasm to organize cellular contents and promote rapid adaptation during stress. To understand how SGs contribute to physiological functions, we used electrochemical measurements to detect electroactive species in SGs. With amperometry, we discovered that reactive oxygen species (ROS) are encapsulated inside arsenite‐induced SGs, and H2O2 is the main species. The release kinetics of H2O2 from single SGs and the number of H2O2 molecules were quantified. The discovery that SGs contain ROS implicates them as communicators of the cellular stresses rather than a simple endpoint. This may explain how SGs regulate cellular metabolism and stress responses. This may also help better understand their cytoprotective functions in pathological conditions associated with SGs such as neurodegenerative diseases (NDs), cancers and viral infections. Arsenite‐induced stress granules isolated from both non‐cancer and cancer cells contain reactive oxygen species as revealed by amperometric measurements with Pt or platinized carbon microelectrodes. This finding supports a role for SGs as signaling hubs and unveils a correlation between SG biology and pathogenesis in neurodegenerative diseases and some cancers.
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影响因子：
9
作者：
通讯作者：
--
DOI：
10.1083/jcb.201302044
发表时间：
2013-04-29
期刊：
The Journal of cell biology
影响因子：
--
作者：
Li YR;King OD;Shorter J;Gitler AD
通讯作者：
Gitler AD
影响因子：
15
作者：
Li, Yun;Hu, Keke;Mirkin, Michael V.
通讯作者：
Mirkin, Michael V.
影响因子：
4
作者：
Lipinski, Simone;Till, Andreas;Rosenstiel, Philip
通讯作者：
Rosenstiel, Philip
影响因子：
4.8
作者：
Gill, Tejpal;Levine, Alan D.
通讯作者：
Levine, Alan D.