Investigating the role of abscisic acid and its catabolites on senescence processes in green asparagus under controlled atmosphere (CA) storage regimes.
Investigating the role of abscisic acid and its catabolites on senescence processes in green asparagus under controlled atmosphere (CA) storage regimes.
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DOI：
10.1016/j.postharvbio.2022.111892
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发表时间：
2022-06
影响因子：
7
通讯作者：
Terry LA
中科院分区：
文献类型：
--
作者：
Anastasiadi M;Collings ER;Terry LA
芦笋（Asparagus officinalis）是一种高度易腐的作物，采后寿命短。虽然已经对气调保鲜技术的应用进行了一些研究，但还没有得到充分的探索，控制芦笋衰老过程的潜在机制也没有得到很好的理解，限制了其商业应用的潜力。本研究的目的是首次研究脱落酸（阿坝）和阿坝催化剂与芦笋在不同气调贮藏条件下衰老的关系。一种传统的CA，由国际可控气氛（伊卡）系统提供，持续供气，LabPods™配有传感器，用于真实的实时监测呼吸率（RR）和呼吸商（RQ），并能够保持既定的CA条件与最低的气体供应要求。遗传变异的作用也进行了研究，包括两个英国生长的芦笋品种'Gijnlim'和'Jaleo'适应不同的气候条件。结果表明，阿坝及其catenorase是目前在空气中存储的矛（对照）在整个存储过程中，无论品种CA相比，浓度显着较高，并与加速衰老过程中观察到的控制样品，如质地的变化，指示矛韧化，变色，糖耗尽和天冬酰胺积累。此外，偏最小二乘回归（PLS-R）适用于这两个品种，成功区分样品的基础上O2和CO2浓度和存储时间，无论是在冷藏和货架期的分离主要是由阿坝和其catenylate驱动。生理生化结果表明，所有三种CA条件下，与空气（对照）相比，（[CA 1] 2.5%O2，3%CO2，[CA 2] 2.5%O2，6%CO2和[CA 3] 2.5%O2，10%CO2）成功地保持了包括质地、颜色、水分含量和视觉外观在内的质量参数更长时间;然而，它们不能完全抑制贮藏末期“梢部分解”（一种生理障碍，也称为梢部腐烂）的发展，这与表明脱落的生物合成和分解代谢途径的激活的菜豆酸浓度的升高相一致。可以得出结论，CA条件可以延迟衰老至少3周（2周冷藏和1周货架期），通过降低代谢率和呼吸商（RQ）内的矛相比，控制，并通过成功地调节阿坝的生物合成和分解代谢途径。阿坝和PA与芦笋的加速衰老过程有关。CA通过抑制阿坝及其代谢产物的积累延长芦笋的贮藏期。与对照相比，低O2和高CO2有助于维持较高的糖含量。LabPods™能够实时监测芦笋的代谢率。
Asparagus (Asparagus officinalis) is a highly perishable crop with a short postharvest life. Although some research has been done on the application of controlled atmosphere (CA), it has not been sufficiently explored and the underlying mechanisms controlling asparagus senescence processes are not well understood, restricting its potential for commercial application. The aim of this study was to investigate for the first time the link between abscisic acid (ABA) and ABA catabolites and senescence in asparagus stored under a range of different CA conditions. Two different set-ups were run in parallel; a traditional CA delivered by an International Controlled Atmosphere (ICA) system with continuous gas supply and LabPods™ fitted with sensors for real time monitoring of respiration rate (RR) and respiratory quotient (RQ) and able to retain established CA conditions with minimum gas supply requirements. The role of genetic variability was also studied by including two UK grown asparagus cultivars ‘Gijnlim’ and ‘Jaleo’ adapted for different climatic conditions. The results indicated that ABA and its catabolites were present in significantly higher concentrations in the air stored spears (control) compared to CA throughout storage, irrespective of cultivar, and were associated with accelerated senescence processes observed in control samples, such as textural changes indicative of spear toughening, discolouration, sugar depletion and asparagine accumulation. Furthermore, partial least squares regression (pls-r) applied for both cultivars, successfully differentiated samples based on O2 and CO2 concentrations and storage duration, both in cold storage and during shelf-life with the separation being driven primarily by ABA and its catabolites. Physiological and biochemical results indicated that all three CA conditions tested ([CA1] 2.5% O2, 3% CO2, [CA2] 2.5% O2, 6% CO2 and [CA3] 2.5% O2, 10% CO2) successfully retained quality parameters including texture, colour, moisture content and visual appearance longer compared to air (control); however, they did not completely suppress the development of ‘tip-breakdown’ (a physiological disorder also known as tip rot) towards the end of storage, which coincided with rising concentrations of phaseic acid indicating an activation of the abscisic biosynthetic and catabolic pathway. It can be concluded that CA conditions can delay senescence for at least 3-weeks (2 weeks cold storage and 1 week shelf-life), by lowering metabolic rate and respiratory quotient (RQ) within the spears compared to control, and through successfully regulating ABA biosynthetic and catabolic pathways. ABA and PA were associated with accelerated senescence processes in asparagus. CA extended asparagus storage life by suppressing ABA and ABA catabolite accumulation. Low O2 and high CO2 helped maintain higher sugar content compared to control. LabPods™ enabled real-time monitoring of asparagus metabolic rates.
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