Microbial Changes Associated With Oral Cavity Cancer Progression.
Microbial Changes Associated With Oral Cavity Cancer Progression.
复制标题
DOI：
10.1002/ohn.211
复制
发表时间：
2023-06
影响因子：
3.4
通讯作者：
Agrawal, Nishant
中科院分区：
文献类型：
--
作者：
Yan, Kenneth;Auger, Samuel;Diaz, Ashley;Naman, Julia;Vemulapalli, Ramya;Hasina, Rifat;Izumchenko, Evgeny;Shogan, Benjamin;Agrawal, Nishant
在口腔鳞状细胞癌的背景下检查口腔微生物组。基础科学研究。学术医疗中心。从因口腔鳞状细胞癌而到手术室接受治疗的患者以及年龄和性别匹配的因无关良性疾病接受手术的对照患者中采集口腔拭子。对从拭子中获得的遗传物质进行16 S核糖体RNA（rRNA）测序。创建细菌rRNA基因文库，并将序列读段分类为分类单位。31名对照患者（17名男性）和35名癌症患者（21名男性）入组。对照组患者的年龄范围为23至89岁（中位数63岁），癌症患者为35至86岁（中位数66岁）。61%的对照组患者和63%的癌症患者是吸烟者。16 S分析表明口腔癌患者中链球菌属显著减少（34.11% vs 21.74%，p = 0.04）。梭杆菌、消化链球菌、细小单胞菌和奈瑟氏球菌的数量也有所增加，这些细菌的丰度与肿瘤的T分期相关。16 S rRNA测序证实了与非癌症对照相比，口腔癌及其进展中细菌种群的变化。我们发现与肿瘤阶段相对应的细菌属的增加-梭菌，消化链球菌，细小单胞菌，奈瑟菌和密螺旋体。这些数据表明，口腔癌创造了一个环境，以促进外来细菌的生长，而不是牵连一个特定的细菌物种在致癌作用。这些细菌可以用作肿瘤进展的潜在标志物，或被询问以更好地表征肿瘤微环境。
To examine the oral microbiome in the context of oral cavity squamous cell carcinoma. Basic science research. Academic medical center. Oral swabs were collected from patients presenting to the operating room for management of oral cavity squamous cell carcinoma and from age- and sex-matched control patients receiving surgery for unrelated benign conditions. 16S ribosomal RNA (rRNA) sequencing was performed on genetic material obtained from swabs. A bacterial rRNA gene library was created and sequence reads were sorted into taxonomic units. Thirty-one control patients (17 males) and 35 cancer patients (21 males) were enrolled. Ages ranged from 23 to 89 (median 63) for control patients and 35 to 86 (median 66) for cancer patients. Sixty-one percent of control patients and 63% of cancer patients were smokers. 16S analyses demonstrated a significant decrease in Streptococcus genera in oral cancer patients (34.11% vs 21.74% of the population, p = .04). Increases in Fusobacterium, Peptostreptococcus, Parvimonas, and Neisseria were also found. The abundance of these bacteria correlated with tumor T-stage. 16S rRNA sequencing demonstrated changes in bacterial populations in oral cavity cancer and its progression compared to noncancer controls. We found increases in bacteria genera that correspond with tumor stage—Fusobacteria, Peptostreptococcus, Parvimonas, Neisseria, and Treponema. These data suggest that oral cancer creates an environment to facilitate foreign bacterial growth, rather than implicating a specific bacterial species in carcinogenesis. These bacteria can be employed as a potential marker for tumor progression or interrogated to better characterize the tumor microenvironment.
登录
查看更多内容
影响因子：
16.6
作者：
Aran D;Camarda R;Odegaard J;Paik H;Oskotsky B;Krings G;Goga A;Sirota M;Butte AJ
通讯作者：
Butte AJ
与喉癌相关的喉部微生物群结构的改变
影响因子：
4.6
作者：
Gong H;Shi Y;Xiao X;Cao P;Wu C;Tao L;Hou D;Wang Y;Zhou L
通讯作者：
Zhou L
影响因子：
5.7
作者：
Nordsmark, M;Bentzen, SM;Overgaard, J
通讯作者：
Overgaard, J
影响因子：
4.4
作者：
Schloss, Patrick D.;Westcott, Sarah L.;Weber, Carolyn F.
通讯作者：
Weber, Carolyn F.
影响因子：
5.2
作者：
Amer A;Galvin S;Healy CM;Moran GP
通讯作者：
Moran GP