Seminal Plasma Antioxidants Are Related to Sperm Cryotolerance in the Horse.
Seminal Plasma Antioxidants Are Related to Sperm Cryotolerance in the Horse.
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DOI：
10.3390/antiox11071279
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发表时间：
2022-06-28
期刊：
影响因子：
--
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中科院分区：
文献类型：
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本研究旨在探讨马精浆中超氧化物歧化酶、谷胱甘肽过氧化物酶、过氧化氢酶和对氧磷酶1型抗氧化剂与非酶类抗氧化剂(Trolox等效抗氧化剂TEAC、铜还原抗氧化剂CUPRAC和血浆铁还原能力FRAP)以及精浆氧化应激指数(OSI)与马精子抗冻融能力的关系。收集精液21份(每人一份)，分成两份：第一份用于采集SP并评估抗氧化剂和OSI的活性水平，第二份用于冷冻保存。解冻后的精子质量参数包括：精子活力、质膜和顶体完整性、线粒体膜电位、细胞内活性氧(ROS)水平和质膜脂质紊乱。根据解冻后的总活动率(TM)和质膜完整性(SYBR14+/PI−)，将精液分级(P<；0.001)分为冷冻性能好(GFE)和冷冻性能差(PFE)两组。GFE中PON1、SOD和TEAC的SP活性水平高于PFE(p<；0.05)，且与精子的某些运动参数、质膜(PON1和TEAC)和顶体(SOD和TEAC)的完整性呈正相关(p<；0.05)。相反，PFE的SP组OSI高于GFE组(p<；0.05)，且与某些精子运动参数、顶体完整性、质膜完整性呈负相关(p<；0.05)，与高质膜脂代谢障碍的精子存活率呈正相关(p<；0.05)。综上所述，SP的酶(PON1和SOD)和非酶(TEAC)抗氧化剂与马精子的耐冷性有关。此外，我们的结果表明，PON1可能是参与该物种ROS控制的主要抗氧化酶之一。需要进一步的研究来证实这些SP-抗氧化剂和OSI用于预测马的精子低温耐受性的潜在用途。
The objective of this study was to determine the relationship of enzymatic (superoxide dismutase, SOD; glutathione peroxidase, GPX; catalase, CAT; and paraoxonase type 1, PON1) and non-enzymatic antioxidants (measured in terms of: Trolox equivalent antioxidant capacity, TEAC; cupric-reducing antioxidant capacity, CUPRAC; and ferric-reducing ability of plasma, FRAP), as well as the oxidative stress index (OSI) in seminal plasma (SP) with the resilience of horse sperm to freeze-thawing. Twenty-one ejaculates (one per individual) were collected and split into two aliquots: the first was used to harvest the SP and assess the activity levels of antioxidants and the OSI, and the second one was cryopreserved. The following post-thaw sperm quality parameters were evaluated: sperm motility, plasma membrane and acrosome integrity, mitochondrial membrane potential, intracellular levels of reactive oxygen species (ROS), and plasma membrane lipid disorder. Based on post-thaw total motility (TM) and plasma membrane integrity (SYBR14+/PI−), ejaculates were hierarchically (p < 0.001) clustered into two groups of good (GFE) and poor (PFE) freezability. The SP activity levels of PON1, SOD, and TEAC were higher (p < 0.05) in GFE than in PFE, showing a positive relationship (p < 0.05) with some sperm motility parameters and with plasma membrane (PON1 and TEAC) and acrosome (SOD and TEAC) integrity. In contrast, OSI was higher (p < 0.05) in the SP of PFE than in that of GFE, and was negatively correlated (p < 0.05) to some sperm motility parameters and to plasma membrane and acrosome integrity, and positively (p < 0.05) to the percentage of viable sperm with high plasma membrane lipid disorder. In conclusion, enzymatic (PON1 and SOD) and non-enzymatic (TEAC) antioxidants of SP are related to horse sperm cryotolerance. In addition, our results suggest that PON1 could be one of the main antioxidant enzymes involved in the control of ROS in this species. Further investigation is needed to confirm the potential use of these SP-antioxidants and OSI to predict sperm cryotolerance in horses.
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DOI：
10.3390/antiox11020417
发表时间：
2022-02-18
期刊：
Antioxidants (Basel, Switzerland)
影响因子：
--
作者：
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通讯作者：
Aurich, Christine
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通讯作者：
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