Impact of Seminal Plasma Antioxidants on Donkey Sperm Cryotolerance.
Impact of Seminal Plasma Antioxidants on Donkey Sperm Cryotolerance.
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精浆抗氧化剂对驴精子低温耐受性的影响。
DOI：
10.3390/antiox11020417
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发表时间：
2022-02-18
期刊：
影响因子：
--
通讯作者：
Barranco I
中科院分区：
文献类型：
--
作者：
Catalán J;Yánez-Ortiz I;Tvarijonaviciute A;González-Arostegui LG;Rubio CP;Yeste M;Miró J;Barranco I
本研究探讨了驴精浆（SP）中抗氧化成分的活性——酶（超氧化物歧化酶（SOD）、过氧化氢酶样（CAT）、谷胱甘肽过氧化物酶样（GPX）和1型对氧酶（PON1））和非酶（以总硫醇、铜还原能力（CUPRAC）、血浆铁还原能力（FRAP）和Trolox等效抗氧化能力（TEAC）衡量）和氧化应激指数（OSI）的活性是否与精子的低温耐量有关。为此，从驴身上收集了15次射精（每人一次），并分成两份。第一个样品用于测定SP中酶促、非酶促抗氧化剂和OSI的活性水平，另一个样品冷冻保存。冷冻保存前，评估精子质量参数（浓度、活力和活力）。解冻后，测定精子活力、质膜完整性、脂质紊乱、线粒体膜电位、活性氧（ROS）和细胞内钙水平。根据解冻后总活力（TM）和完整质膜（SYBR14+/PI−）精子的百分比，通过聚类分析将样品分为抗冻性好（GFE）或抗冻性差（PFE）射精。GFE的SP酶活性（SOD、PON1）和非酶抗氧化剂（CUPRAC、FRAP和TEAC）水平高于PFE (p < 0.05)， SP- osi活性水平高于GFE （p < 0.05）。此外，SOD、PON1、GPX、CUPRAC、FRAP和TEAC活性水平与解冻后精子活力和质膜完整性呈正相关（p < 0.05），与细胞内ROS水平负相关（p < 0.05）。SP-OSI与解冻后精子质量参数呈负相关（p < 0.05），与细胞内ROS水平呈正相关（p < 0.05）。由此可见，驴SP抗氧化剂与精子低温耐受性有关，抗氧化剂PON1、SOD、CUPRAC、FRAP、TEAC以及SP- osi的测定可作为精子低温耐受性的标志物。进一步研究这些抗氧化剂和SP-OSI与精子低温耐受性的关系及其作为冷冻标志物的潜在用途是有必要的。
This study investigated whether the activities of the antioxidant components of donkey seminal plasma (SP)—both enzymatic (superoxide dismutase (SOD), catalase-like (CAT), glutathione peroxidase-like (GPX), and paraoxonase type 1 (PON1)) and non-enzymatic (measured in terms of total thiol, copper-reducing antioxidant capacity (CUPRAC), ferric-reducing ability of plasma (FRAP), and Trolox equivalent antioxidant capacity (TEAC))—and oxidative stress index (OSI) are related to sperm cryotolerance. For this purpose, 15 ejaculates from jackasses (one per individual) were collected and split into two aliquots. The first one was used for measuring the activities levels of enzymatic and non-enzymatic antioxidants and OSI in SP, whereas the other aliquot was cryopreserved. Before cryopreservation, sperm quality parameters (concentration, motility, and viability) were evaluated. After thawing, sperm motility, plasma membrane integrity, lipid disorder, mitochondrial membrane potential, reactive oxygen species (ROS), and calcium intracellular levels were also determined. Based on the percentages of total motility (TM) and of sperm with an intact plasma membrane (SYBR14+/PI−) after thawing, samples were classified as good-freezability (GFE) or poor-freezability (PFE) ejaculates through cluster analyses. The SP activity levels of enzymatic (SOD and PON1) and non-enzymatic antioxidants (CUPRAC, FRAP, and TEAC) were higher (p < 0.05) in GFE than in PFE, whereas SP-OSI was higher (p < 0.05) in PFE than in GFE. In addition, the activity levels of SOD, PON1, GPX, CUPRAC, FRAP, and TEAC were positively (p < 0.05) related to post-thaw sperm motility and plasma membrane integrity and negatively to intracellular ROS levels. The SP-OSI was negatively correlated (p < 0.05) to post-thaw sperm quality parameters and positively to intracellular ROS levels. It can thus be concluded that donkey SP antioxidants are related to sperm cryotolerance and that measurements of antioxidants PON1, SOD, CUPRAC, FRAP, and TEAC, as well as SP-OSI, could be used as markers of sperm cryotolerance. Further research addressing the relationship of these antioxidants and SP-OSI with sperm cryotolerance and their potential use as freezing markers is warranted.
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影响因子：
7.4
作者：
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