Integrins control tissue morphogenesis and homeostasis by sustaining the different types of intracellular actin networks
Integrins control tissue morphogenesis and homeostasis by sustaining the different types of intracellular actin networks
复制标题
整合素通过维持不同类型的细胞内肌动蛋白网络来控制组织形态发生和稳态
DOI：
10.1101/639427
复制
发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Mateos C
中科院分区：
文献类型：
--
作者：
Mateos C
肌动球蛋白细胞骨架产生的力是组织形状形成的关键因素。在细胞内，肌动球蛋白细胞骨架以不同类型的网络组织，每一种网络都有不同的作用。此外，虽然它们通常定位于细胞的精确区域，但它们很少是独立的，而且它们的动态常常相互影响。事实上，一个给定结构的重组可以促进另一个结构的形成，这种转换控制着许多形态发生过程。此外，在分化组织中维持特定的肌动球蛋白网络组织可能同样重要。如果不这样做，可能会导致不希望的细胞状态转变，这反过来又会对组织的内稳态产生严重后果。然而，在分化组织中，在形态发生或维持过程中，肌动球蛋白网络之间的受控转换机制仍然知之甚少。在这里，我们使用果蝇滤泡上皮来证明由整合素介导的细胞- ecm相互作用对于这些上皮细胞中不同的肌动球蛋白网络的建立和维持是必要的。一组卵泡细胞中整合素的消除导致f -肌动蛋白水平和细胞内肌动球蛋白网络物理特性的变化。整合素突变的卵泡细胞减少了基底应力纤维的数量。它们还表现出皮层f -肌动蛋白水平和张力的增加，这干扰了基底表面的正常生长。最后，整合素的克隆消除也会引发邻近野生型细胞的非自主行为变化，这些细胞现在重组它们的肌动蛋白细胞骨架，并扩散和覆盖突变细胞。基于这些结果，我们提出整合素介导的细胞- ecm相互作用通过保留不同类型的细胞内肌动蛋白网络来调节上皮细胞的形态发生和稳态。
Forces generated by the actomyosin cytoskeleton are key contributors to the generation of tissue shape. Within the cell, the actomyosin cytoskeleton organizes in different types of networks, each of them performing distinct roles. In addition, although they normally localize to precise regions of the cells, they are rarely independent and often their dynamics influence each other. In fact, the reorganization of a given structure can promote the formation of another, conversions that govern many morphogenetic processes. In addition, maintenance of a specific actomyosin network organization in a differentiated tissue might be equally important. Failure to do so could lead to undesired cell state transitions, which in turn would have drastic consequences on the homeostasis of the tissue. Still, little is known about the mechanisms that ensure controlled transitions between actomyosin networks during morphogenesis or their maintenance in a differentiated tissue. Here, we use theDrosophilafollicular epithelium to show that cell-ECM interactions mediated by integrins are necessary for the establishment and maintenance of the different actomyosin networks present in these epithelial cells. Elimination of integrins in a group of follicle cells results in changes in the F-actin levels and physical properties of their intracellular actomyosin networks. Integrin mutant follicle cells have reduced number of basal stress fibers. They also show increased cortical F-actin levels and tension, which interferes with proper basal surface growth. Finally, clonal elimination of integrins also triggers non-autonomous behavioural changes in neighbouring wild types cells, which now reorganize their actin cytoskeleton and spread and overlay the mutant ones. Based on these results, we propose that cell-ECM interactions mediated by integrins regulate epithelia morphogenesis and homesostasis by preserving the different types of intracellular actin networks.
登录
查看更多内容
影响因子：
3.3
作者：
Baird MA;Billington N;Wang A;Adelstein RS;Sellers JR;Fischer RS;Waterman CM
通讯作者：
Waterman CM
肌球体突变等位基因的表征，肌球体是编码果蝇 PS 整联蛋白 β 亚基的基因。
影响因子：
3.3
作者：
Bunch,TA;Salatino,R;Engelsgjerd,MC;Mukai,L;West,RF;Brower,DL
通讯作者：
Brower,DL
影响因子：
2.7
作者：
Kolahi, K. S.;White, P. F.;Shreter, D. M.;Classen, A-K.;Bilder, D.;Mofrad, M. R. K.
通讯作者：
Mofrad, M. R. K.
影响因子：
0.7
作者：
Fernandez-Minan, Ana;Cobreros, Laura;Martin-Bermudo, Maria D.
通讯作者：
Martin-Bermudo, Maria D.
影响因子：
9.2
作者：
Kunda, Patricia;Pelling, Andrew E.;Baum, Buzz
通讯作者：
Baum, Buzz