Quantitative analysis of epithelial morphogenesis in Drosophila oogenesis: New insights based on morphometric analysis and mechanical modeling.
Quantitative analysis of epithelial morphogenesis in Drosophila oogenesis: New insights based on morphometric analysis and mechanical modeling.
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DOI：
10.1016/j.ydbio.2009.04.028
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发表时间：
2009-07-15
影响因子：
2.7
通讯作者：
Mofrad, M. R. K.
中科院分区：
文献类型：
--
作者：
Kolahi, K. S.;White, P. F.;Shreter, D. M.;Classen, A-K.;Bilder, D.;Mofrad, M. R. K.
关键词：
上皮形态发生过程在动物发育中普遍存在，但关于上皮组织的形成机制还有很多有待了解。果蝇的卵泡细胞（FC）上皮包裹着正在生长的种系，是研究上皮发育基本要素的绝佳系统。在卵子发生的第 8 至 10 阶段，FC 上皮在简单的几何形状（立方形、柱状和鳞状）之间转变，并在称为后迁移、鳞状细胞扁平化和主体细胞柱状化的过程中重新分布细胞群。在这里，我们对这些知之甚少的事件进行了定量形态分析，以便建立参数并界定调节转变的潜在过程。我们的研究结果迫使人们对这些现象的公认观点进行了惊人的修正，表明后部迁移不涉及FC运动，柱状细胞顶端收缩在FC形态发生中没有作用，并且鳞状细胞扁平化可能是对种系生长的顺从反应。我们利用涉及有限元计算技术的机械建模来证明随时间变化的粘弹性特性和生长足以解释大部分 FC 形态变化。
The process of epithelial morphogenesis is ubiquitous in animal development, but much remains to be learned about the mechanisms that shape epithelial tissues. The follicle cell (FC) epithelium encapsulating the growing germline of Drosophila is an excellent system to study fundamental elements of epithelial development. During stages 8 to 10 of oogenesis, the FC epithelium transitions between simple geometries - cuboidal, columnar and squamous – and redistributes cell populations in processes described as posterior migration, squamous cell flattening and main body cell columnarization. Here we have carried out a quantitative morphometric analysis of these poorly understood events in order to establish the parameters of and delimit the potential processes that regulate the transitions. Our results compel a striking revision of accepted views of these phenomena, by showing that posterior migration does not involve FC movements, that there is no role for columnar cell apical constriction in FC morphogenesis, and that squamous cell flattening may be a compliant response to germline growth. We utilize mechanical modeling involving finite element computational technologies to demonstrate that time-varying viscoelastic properties and growth are sufficient to account for the bulk of the FC morphogenetic changes.
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影响因子：
9.2
作者：
Wang, Ying;Riechmann, Veit
通讯作者：
Riechmann, Veit
DOI：
10.1115/1.2895713
发表时间：
1994-05-01
影响因子：
1.7
作者：
BRODLAND, GW;CLAUSI, DA
通讯作者：
CLAUSI, DA
影响因子：
2.7
作者：
Lee, Jen-Yi;Harland, Richard M.
通讯作者：
Harland, Richard M.
影响因子：
9.2
作者：
Bateman, J;Reddy, RS;Van Vactor, D
通讯作者：
Van Vactor, D
影响因子：
11.8
作者：
Prasad, Mohit;Montell, Denise J.
通讯作者：
Montell, Denise J.