Immunohistochemical and proteomic evaluation of nuclear ubiquitous casein and cyclin-dependent kinases substrate in invasive ductal carcinoma of the breast.
Immunohistochemical and proteomic evaluation of nuclear ubiquitous casein and cyclin-dependent kinases substrate in invasive ductal carcinoma of the breast.
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DOI：
10.1155/2009/919645
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发表时间：
2009
影响因子：
--
通讯作者：
Wiśniewski JR
中科院分区：
文献类型：
--
作者：
Ziółkowski P;Gamian E;Osiecka B;Zougman A;Wiśniewski JR
核普遍存在的酪蛋白和细胞周期蛋白依赖性激酶底物 (NUCKS) 是功能未知的 27kDa 染色体蛋白。其氨基酸组成及其 DNA 结合域的结构类似于高迁移率 A 组 HMGA 蛋白。 HMGA 蛋白与多种恶性肿瘤相关。由于 HMGA 表达的变化被认为是肿瘤进展的标志，因此 NUCKS 表达的类似变化可能成为乳腺癌诊断和预后的有用工具。为了鉴定和分析 NUCKS，我们使用蛋白质组学和组织化学方法。通过质谱分析对正常和乳腺癌患者匹配样本进行分析，发现导管乳腺癌蛋白质提取物中 NUCKS 水平升高。我们引出了针对 NUCKS 的特异性抗体，并将其用于乳腺癌浸润性导管癌的免疫组织化学分析。我们发现 NUCKS 在 84.3% 的癌细胞中高表达。我们认为 NUCKS 的这种过度表达可以在乳腺癌生物学中发挥重要作用。
Nuclear ubiquitous casein and cyclin-dependent kinases substrate (NUCKS) is 27 kDa chromosomal protein of unknown function. Its amino acid composition as well as structure of its DNA binding domain resembles that of high-mobility group A, HMGA proteins. HMGA proteins are associated with various malignancies. Since changes in expression of HMGA are considered as marker of tumor progression, it is possible that similar changes in expression of NUCKS could be useful tool in diagnosis and prognosis of breast cancer. For identification and analysis of NUCKS we used proteomic and histochemical methods. Analysis of patient-matched samples of normal and breast cancer by mass spectrometry revealed elevated levels of NUCKS in protein extracts from ductal breast cancers. We elicited specific antibodies against NUCKS and used them for immunohistochemistry in invasive ductal carcinoma of breast. We found high expression of NUCKS in 84.3% of cancer cells. We suggest that such overexpression of NUCKS can play significant role in breast cancer biology.
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影响因子：
--
作者：
Hennig, Y;Rogalla, P;Bullerdiek, J
通讯作者：
Bullerdiek, J
DOI：
10.1046/j.1524-4741.2001.99124.x
发表时间：
2001-09-01
期刊：
The breast journal
影响因子：
--
作者：
Miller, N A;Chapman, J A;Hanna, W M
通讯作者：
Hanna, W M
影响因子：
3.9
作者：
Pierantoni, GM;Finell, P;Larizza, L
通讯作者：
Larizza, L
影响因子：
3.8
作者：
Staats, B;Bonk, U;Bullerdiek, J
通讯作者：
Bullerdiek, J
影响因子：
4.1
作者：
Pierantoni, GM;Agosti, V;Fusco, A
通讯作者：
Fusco, A