All subtypes of the Pmp adhesin family are implicated in chlamydial virulence and show species-specific function.
All subtypes of the Pmp adhesin family are implicated in chlamydial virulence and show species-specific function.
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DOI：
10.1002/mbo3.186
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发表时间：
2014-08
期刊：
影响因子：
3.4
通讯作者：
Hegemann, Johannes H.
中科院分区：
文献类型：
--
作者：
Becker, Elisabeth;Hegemann, Johannes H.
细菌病原体沙眼衣原体和肺炎衣原体是专性细胞内寄生虫，可引起许多严重疾病，并可感染人类各种细胞类型。衣原体感染可能是由细菌外膜蛋白OmcB与宿主细胞糖胺聚糖（GAGs）结合而引发的。在这里，我们发现沙眼衣原体多态膜蛋白（Pmp）家族的所有9个成员介导人上皮细胞和内皮细胞的粘附。重要的是，感染性颗粒暴露于可溶性重组Pmps可阻断后续感染，从而暗示整个蛋白家族在感染过程中的重要功能。对重组Pmps或Pmp与OmcB的组合进行的类似实验表明，所有Pmps都可能在不同于OmcB- gag途径的粘附途径中起作用。最后，我们提供证据表明沙眼衣原体和肺炎衣原体的Pmps表现出物种和组织特异性。这些发现表明沙眼衣原体Pmps参与感染的初始阶段，并提示它们可能与一种受体相互作用，而不是最近在肺炎衣原体中发现的表皮生长因子受体。
The bacterial pathogens Chlamydia trachomatis and C. pneumoniae are obligate intracellular parasites, cause a number of serious diseases, and can infect various cell types in humans. Chlamydial infections are probably initiated by binding of the bacterial outer membrane protein OmcB to host cell glycosaminoglycans (GAGs). Here, we show that all nine members of the polymorphic membrane protein (Pmp) family of C. trachomatis mediate adhesion to human epithelial and endothelial cells. Importantly, exposure of infectious particles to soluble recombinant Pmps blocks subsequent infection, thus implicating an important function of the entire protein family in the infection process. Analogous experiments with pairs of recombinant Pmps or a combination of Pmp and OmcB revealed that all Pmps probably act in an adhesion pathway that is distinct from the OmcB-GAG pathway. Finally, we provide evidence that the Pmps of C. trachomatis and C. pneumoniae exhibit species and tissue specificity. These findings argue for the involvement of C. trachomatis Pmps in the initial phase of infection and suggest that they may interact with a receptor other than the epidermal growth factor receptor recently identified for their counterparts in C. pneumoniae.
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