Versatile potentiometric metabolite sensing without dioxygen interference.
Versatile potentiometric metabolite sensing without dioxygen interference.
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DOI：
10.1016/j.bios.2021.113888
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发表时间：
2022-04-01
影响因子：
12.6
通讯作者：
Dick JE
中科院分区：
文献类型：
--
作者：
Walker NL;Dick JE
电化学生物传感器领域一直由电流型和伏安型传感器主导，然而，这些传感器在其信号对电极尺寸的依赖性方面受到很大限制。开路电位传感器由于其信号对电极尺寸不敏感而成为替代品。在这里，我们提出了第二代生物传感器，使用改性壳聚糖水凝胶捕获脱氢酶或其他氧化还原酶的利益。壳聚糖用所需的电子介体修饰，使得在分析物存在下，酶将氧化或还原介体，从而改变测量的界面电位。使用上述设计，我们证明了一种快速筛选方法，适当的酶介体对开路电位法的基础上，以及使用两种脱氢酶（FADGDH和ADH）和过氧化物酶的生物传感器设计的功效。使用1，2-萘醌作为FADGDH的介体，实现了0.1-50 mM葡萄糖的动态范围。我们还证明了易于制造和修改，寿命≥ 28天，对干扰物不敏感，微型化到微米级，以及在酶的天然辅因子存在下的传感器功效。这些结果为电位生物传感器的广泛应用奠定了基础，用于生物相关系统中的各种分析物，其中氧气可以是一种替代物。
The field of electrochemical biosensors has been dominated by amperometric and voltammetric sensors; however, these are limited greatly in their signal dependence on electrode size. Open circuit potentiometric sensors are emerging as an alternative due to their signal insensitivity to electrode size. Here, we present a second-generation biosensor that uses a modified chitosan hydrogel to entrap a dehydrogenase or other oxidoreductase enzyme of interest. The chitosan is modified with a desired electron mediator such that in the presence of the analyte, the enzyme will oxidize or reduce the mediator, thus altering the measured interfacial potential. Using the above design, we demonstrate a swift screening method for appropriate enzyme-mediator pairs based on open circuit potentiometry, as well as the efficacy of the biosensor design using two dehydrogenase enzymes (FADGDH and ADH) and peroxidase. Using 1,2-naphthoquinone as the mediator for FADGDH, dynamic ranges from 0.1–50 mM glucose are achieved. We additionally demonstrate the ease of fabrication and modification, a lifetimes of ≥ 28 days, insensitivity to interferents, miniaturization to the microscale, and sensor efficacy in the presence of the enzyme’s natural cofactor. These results forge a foundation for the generalized use of potentiometric biosensors for a wide variety of analytes within biologically-relevant systems where oxygen can be an interferent.
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DOI：
10.1126/science.aad5168
发表时间：
2016-06-17
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Cambronne XA;Stewart ML;Kim D;Jones-Brunette AM;Morgan RK;Farrens DL;Cohen MS;Goodman RH
通讯作者：
Goodman RH
影响因子：
3
作者：
Elgrishi, Noemie;Rountree, Kelley J.;Dempsey, Jillian L.
通讯作者：
Dempsey, Jillian L.
影响因子：
2.9
作者：
Luo, XL;Xu, JJ;Chen, HY
通讯作者：
Chen, HY
影响因子：
6.2
作者：
Baez, Jhonattan F.;Compton, Matthew;Andrade, Francisco J.
通讯作者：
Andrade, Francisco J.
影响因子：
8.8
作者：
Guadarrama-Fernandez, Leonor;Novell, Marta;Andrade, Francisco J.
通讯作者：
Andrade, Francisco J.