Comprehensive two-dimensional APTES-decorated MCF7-cell membrane chromatographic system for characterizing potential anti-breast-cancer components from Yuanhu-Baizhi herbal medicine pair.
Comprehensive two-dimensional APTES-decorated MCF7-cell membrane chromatographic system for characterizing potential anti-breast-cancer components from Yuanhu-Baizhi herbal medicine pair.
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全面的二维 APTES 修饰的 MCF7 细胞膜色谱系统用于表征元胡-白芷草药对中潜在的抗乳腺癌成分
DOI：
10.1016/j.jfda.2017.11.010
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发表时间：
2018-04
影响因子：
3.6
通讯作者：
Hong, Zhan-Ying
中科院分区：
文献类型：
--
作者：
Wang, Xiao-Yu;Ding, Xuan;Yuan, Yong-Fang;Zheng, Le-Yi;Cao, Yan;Zhu, Zhen-Yu;Zhang, Guo-Qing;Chai, Yi-Feng;Chen, Xiao-Fei;Hong, Zhan-Ying
关键词：
元胡和白芷（元胡-白芷）草药对已经使用了数千年，并且据报道在最近的癌症治疗中具有潜在的活性。但确切的活性成分或馏分仍不清楚。本研究建立了一种新的二维（2D）3-氨丙基三乙氧基硅烷（APTES）修饰的MCF 7-细胞膜色谱（CMC）/capcell-C18柱/飞行时间质谱系统，用于筛选元胡-白芷对的潜在活性成分和澄清活性组分。将APTES修饰在硅胶表面，使其在戊二醛的作用下提供一个氨基与细胞膜片段共价连接，以提高MCF 7 CMC柱的稳定性和柱寿命。二维MCF 7-CMC体系具有良好的分离和鉴定能力。筛选结果表明，保留成分主要来自元胡生物碱类成分（12个化合物）和白芷香豆素类成分（10个化合物），揭示了元胡-白芷药对的活性部位。以氧化苦参碱、原阿片碱、小檗碱、蛇床子素、异茴芹素和棕榈酸为代表成分，测定其对细胞增殖的IC_（50）分别为38.17 μM、29.45 μM、45.42 μM、132.7 μM、156.8 μM和90.5 μM。细胞凋亡实验表明，药物主要是通过诱导细胞凋亡来发挥药效的。此外，协同试验结果表明，oxoglaucine（从元胡生物碱的代表）和isopimpinellin（从白芷香豆素的代表）与GFT显示出显着的协同效应，表明这些成分可能作用于其他膜受体。所提出的2D CMC系统还可以配备有用于进一步应用的其他单元。此外，后续的细胞增殖实验、细胞凋亡实验和协同实验等体外实验策略，也为中药活性部位的确定提供了一种可行的方法。
Rhizoma corydalis and Radix Angelicae Dahurica (Yuanhu–Baizhi) herbal medicine pair has been used for thousands of years and has been reported to be potentially active in recent cancer therapy. But the exact active components or fractions remain unclear. In this study, a new comprehensive two-dimensional (2D) 3-aminopropyltriethoxysilane (APTES)-decorated MCF7-cell membrane chromatography (CMC)/capcell-C18 column/time-of-flight mass spectrometry system was established for screening potential active components and clarifying the active fraction of Yuanhu–Baizhi pair. APTES was modified on the surface of silica, which can provide an amino group to covalently link cell membrane fragments with the help of glutaraldehyde in order to improve the stability and column life span of the MCF7 CMC column. The comprehensive 2D MCF7-CMC system showed good separation and identification abilities. Our screen results showed that the retention components are mainly from the alkaloids in Yuanhu (12 compounds) and the coumarins (10 compounds) in Baizhi, revealing the active fractions of Yuanhu–Baizhi herbal medicine pair. Oxoglaucine, protopine, berberine, osthole, isopimpinellin and palmitic acid were selected as typical components to test the effects on cell proliferation and their IC50 were calculated as 38.17 μM, 29.45 μM, 45.42 μM, 132.7 μM, 156.8 μM and 90.5 μM respectively. Cell apoptosis assay showed that the drug efficacy was obtained mainly through inducing cell apoptosis. Furthermore, a synergistic assay results demonstrated that oxoglaucine (representative of alkaloids from Yuanhu) and isopimpinellin (representative of coumarins from Baizhi) showed significant synergistic efficacy with GFT, indicating that these components may act on other membrane receptors. The proposed 2D CMC system could also be equipped with other cells for further applications. Besides, the follow-up in-vitro experimental strategy using cell proliferation assay, cell apoptosis assay and synergistic assay proved to be a practical way to confirm the active fractions of herbal medicine.
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影响因子：
3.8
作者：
Moerkens M;Zhang Y;Wester L;van de Water B;Meerman JH
通讯作者：
Meerman JH
DOI：
10.1155/2013/658531
发表时间：
2013
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Xu H;Tao Y;Lu P;Wang P;Zhang F;Yuan Y;Wang S;Xiao X;Yang H;Huang L
通讯作者：
Huang L
影响因子：
7.9
作者：
Chen, Yuetao;Cao, Yu;Wang, Siwang
通讯作者：
Wang, Siwang
DOI：
10.4314/ajtcam.v11i2.28
发表时间：
2014-01-01
影响因子：
--
作者：
He, Kai;Gao, Jian-Li
通讯作者：
Gao, Jian-Li
细胞膜色谱法在中药分析中的最新进展
DOI：
10.1016/j.jpba.2014.05.021
发表时间：
2014-12-01
影响因子：
3.4
作者：
Hou, Xiaofang;Wang, Sicen;He, Langchong
通讯作者：
He, Langchong