Serum exosome microRNA panel as a noninvasive biomarker for molecular diagnosis of fulminant myocarditis.
Serum exosome microRNA panel as a noninvasive biomarker for molecular diagnosis of fulminant myocarditis.
复制标题
血清外泌体 microRNA 组合作为暴发性心肌炎分子诊断的无创生物标志物
DOI：
10.1016/j.omtm.2020.11.006
复制
发表时间：
2021-03-12
期刊：
影响因子：
--
通讯作者：
Lv K
中科院分区：
文献类型：
--
作者：
Zhang Y;Li X;Wang D;Jiang X;Zhang M;Lv K
外泌体衍生的microRNAs （miRNAs）是潜在的诊断性生物标志物。然而，它们作为暴发性心肌炎（FM）的诊断性生物标志物的有效性知之甚少。本研究旨在探索血清外泌体mirna作为FM诊断的潜在生物标志物。收集99例FM患者、32例非暴发性心肌炎（NFM）患者和105例健康对照（hc）的外周血样本。采用新一代测序技术测定血清外泌体的miRNA表达谱，并通过定量逆转录酶聚合酶链反应进一步分析差异表达的miRNA。采用训练队列（n = 120）构建logistic回归模型，然后采用独立队列（n = 106）进行验证。用受者工作特征曲线下面积评价诊断准确性。在FM患者中，hsa-miR-30a、hsa-miR-192、hsa-miR-146a、hsa-miR-155和hsa-miR-320a被证实是显著和差异表达的候选物，可以作为FM诊断的潜在标记物。此外，来自多变量logistic回归模型的miRNA面板（hsa-miR-155和hsa-miR-320a）在FM诊断中显示出很高的准确性，并且能够将FM与hc和NFM区分开来。此外，miRNA组合的诊断价值大于CRP和cTn单独或联合的诊断价值。miRNA面板为FM提供了出色的诊断能力。本研究调查了FM患者血清中外泌体来源的miRNA谱，发现用于FM诊断的miRNA面板在区分FM与hc和NFM方面具有很高的准确性。我们的研究结果支持循环外泌体miRNA谱可以作为FM的非侵入性生物标志物的假设。
Exosome-derived microRNAs (miRNAs) are potential diagnostic biomarkers. However, little is known about their effectiveness as diagnostic biomarkers of fulminant myocarditis (FM). This study aimed to explore serum exosomal miRNAs as potential biomarkers for FM diagnosis. Peripheral blood samples were collected from 99 patients with FM, 32 patients with nonfulminant myocarditis (NFM), and 105 healthy controls (HCs). The miRNA expression profiles of serum exosomes were determined using next-generation sequencing, and differentially expressed miRNAs were further analyzed by quantitative reverse transcriptase polymerase chain reaction. A logistic regression model was constructed using a training cohort (n = 120) and then validated using an independent cohort (n = 106). The area under the receiver operating characteristic curve was used to evaluate diagnostic accuracy. In FM patients, hsa-miR-30a, hsa-miR-192, hsa-miR-146a, hsa-miR-155, and hsa-miR-320a were validated as significantly and differentially expressed candidates that could serve as potential markers for diagnosing FM. In addition, the miRNA panel (hsa-miR-155 and hsa-miR-320a) from the multivariate logistic regression model demonstrated high accuracy in the diagnosis of FM and was able to distinguish FM from HCs and NFM. Moreover, the diagnostic value of the miRNA panel was greater than that of CRP and cTn alone or together. The miRNA panel provided the excellent diagnostic capability for FM. This study investigated the exosome-derived miRNA profiles in serum from patients with FM and found that the miRNA panel for FM diagnosis exhibited high accuracy in distinguishing FM from HCs and NFM. Our results support the hypothesis that circulating exosomal miRNA profiles can serve as noninvasive biomarkers of FM.
登录
查看更多内容
microRNA分析：方法和考虑因素。
DOI：
10.1038/nrg3198
发表时间：
2012-04-18
期刊：
Nature reviews. Genetics
影响因子：
--
作者：
Pritchard CC;Cheng HH;Tewari M
通讯作者：
Tewari M
影响因子：
2.1
作者：
Gidlöf O;Smith JG;Miyazu K;Gilje P;Spencer A;Blomquist S;Erlinge D
通讯作者：
Erlinge D
影响因子：
37.8
作者：
Ammirati, Enrico;Cipriani, Manlio;Frigerio, Maria
通讯作者：
Frigerio, Maria
影响因子：
--
作者：
Corsten, Maarten F.;Dennert, Robert;Schroen, Blanche
通讯作者：
Schroen, Blanche
影响因子：
3.7
作者：
Gallo A;Tandon M;Alevizos I;Illei GG
通讯作者：
Illei GG