Prevalent ciliate symbiosis on copepods: high genetic diversity and wide distribution detected using small subunit ribosomal RNA gene.
Prevalent ciliate symbiosis on copepods: high genetic diversity and wide distribution detected using small subunit ribosomal RNA gene.
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桡足类中普遍存在的纤毛虫共生：利用小亚基核糖体 RNA 基因检测到高遗传多样性和广泛分布
DOI：
10.1371/journal.pone.0044847
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Lin S
中科院分区：
文献类型：
--
作者：
Guo Z;Liu S;Hu S;Li T;Huang Y;Liu G;Zhang H;Lin S
为了了解桡足类共生纤毛虫的遗传多样性和分布，以及与海洋环境中寄主的进化关系，我们建立了基于小亚基核糖体RNA基因(18S RDNA)的分子方法，并对桡足类共生纤毛虫的遗传多样性和基因分布进行了研究。在太平洋和大西洋6个地点采集的6科10种桡足类动物中，有9种含有纤毛虫共生体。对获得的391个纤毛虫18S rDNA序列的系统发育分析表明，有7个类群(核心群)，其中6个(占所有序列的99%)属于Apostomatida亚纲，另一个与富围纤毛虫Vorticella gracilis聚在一起。在Apostomatida类群中，类群III与Vampyrofleya pelagica基本相同，其他5个类群代表与Vampyrofreya/Gymnoinioides/Hyalophysa相近的未记录纤毛虫。在所获得的纤毛虫序列中，VI组纤毛虫在所有的桡足类物种中均有发现，其中中华哲水蚤(Calanus Sinicus)的纤毛虫数量最多，表明它们是与桡足类普遍相关的优势共生纤毛虫。相反，一些纤毛虫序列只在所研究的部分桡足类中发现，这表明纤毛虫的寄主选择性和地理分化，这需要更广泛的采样来进一步验证。我们的结果揭示了海洋桡足类共生纤毛虫的广泛存在和高度的遗传多样性，并强调了在全球范围内系统研究这些纤毛虫基于寄主和地理的遗传分化和生态作用的必要性。
Toward understanding the genetic diversity and distribution of copepod-associated symbiotic ciliates and the evolutionary relationships with their hosts in the marine environment, we developed a small subunit ribosomal RNA gene (18S rDNA)-based molecular method and investigated the genetic diversity and genotype distribution of the symbiotic ciliates on copepods. Of the 10 copepod species representing six families collected from six locations of Pacific and Atlantic Oceans, 9 were found to harbor ciliate symbionts. Phylogenetic analysis of the 391 ciliate 18S rDNA sequences obtained revealed seven groups (ribogroups), six (containing 99% of all the sequences) belonging to subclass Apostomatida, the other clustered with peritrich ciliate Vorticella gracilis. Among the Apostomatida groups, Group III were essentially identical to Vampyrophrya pelagica, and the other five groups represented the undocumented ciliates that were close to Vampyrophrya/Gymnodinioides/Hyalophysa. Group VI ciliates were found in all copepod species but one (Calanus sinicus), and were most abundant among all ciliate sequences obtained, indicating that they are the dominant symbiotic ciliates universally associated with copepods. In contrast, some ciliate sequences were found only in some of the copepods examined, suggesting the host selectivity and geographic differentiation of ciliates, which requires further verification by more extensive sampling. Our results reveal the wide occurrence and high genetic diversity of symbiotic ciliates on marine copepods and highlight the need to systematically investigate the host- and geography-based genetic differentiation and ecological roles of these ciliates globally.
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影响因子：
4.4
作者：
Doherty, Mary;Tamura, Maiko;Katz, Laura A.
通讯作者：
Katz, Laura A.
DOI：
10.1111/j.1550-7408.1973.tb00865.x
发表时间：
1973-01-01
期刊：
JOURNAL OF PROTOZOOLOGY
影响因子：
--
作者：
BRADBURY, PC;CLAMP, JC
通讯作者：
CLAMP, JC
影响因子：
0.8
作者：
Jayasree, L;Janakiram, P;Madhavi, R
通讯作者：
Madhavi, R
影响因子：
11
作者：
Brate, Jon;Logares, Ramiro;Shalchian-Tabrizi, Kamran
通讯作者：
Shalchian-Tabrizi, Kamran
影响因子：
1.3
作者：
BASSON, L;VANAS, JG
通讯作者：
VANAS, JG