Alterations in Urobiome in Patients With Bladder Cancer and Implications for Clinical Outcome: A Single-Institution Study.
Alterations in Urobiome in Patients With Bladder Cancer and Implications for Clinical Outcome: A Single-Institution Study.
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膀胱癌患者尿生物组的变化及其对临床结果的影响：一项单机构研究
DOI：
10.3389/fcimb.2020.555508
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发表时间：
2020
影响因子：
5.7
通讯作者：
Wu P
中科院分区：
文献类型：
--
作者：
Zeng J;Zhang G;Chen C;Li K;Wen Y;Zhao J;Wu P
Numerous studies indicate that resident microbiome exists in urine of healthy individuals and dysbiosis of the urobiome (urinary microbiome) may be associated with pathological conditions. This study was performed to characterize the alterations in urobiome and explore its implications of clinical outcome in male patients with bladder cancer. 62 male patients with bladder cancer and 19 non-neoplastic controls were recruited. The follow-up study cohort included 40 patients who were diagnosed with non-muscle invasive bladder cancer (NMIBC) and underwent transurethral resection of bladder tumor (TURBT). Mid-stream urine samples were collected from all the participants the day before cystoscopy. DNA was extracted from urine pellet samples and processed for high throughput 16S rRNA amplicon sequencing of the V4 region using Illumina MiSeq. Sequencing reads were filtered using QIIME and clustered using UPARSE. We found bacterial richness indices (Observed Species index, Chao1 index, Ace index; all P < 0.01) increased in cancer group when compared with non-neoplastic group, while there were no differences in Shannon and Simpson index between two groups. During a median follow-up time of 12 (5.25–25) months, 5/40 (12.5%)of the patients developed recurrence and no patient suffered from progression to muscle-invasive disease. Species diversity of the microbiome was significantly higher in the recurrence group compared with non-recurrence group in patients with NMIBC after TURBT. The LEfSe analysis demonstrated that 9 genera were increased (e.g., Micrococcus and Brachybacterium) in recurrence group. To our knowledge we report the relative comprehensive study to date of the male bladder cancer urinary microbiome and its relationship to pathogenesis and clinical outcomes. Given our preliminary data, additional studies evaluating the urine microbiome in relation to clinical outcomes are warranted to improve our understanding of tumor recurrence after TURBT.
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影响因子：
4
作者：
Liu, Fei;Liu, Anwei;Xu, Chuanliang
通讯作者：
Xu, Chuanliang
基于多个数据集的膀胱癌共同核心细菌生物标志物
影响因子：
--
作者：
Mai, Guoqin;Chen, Limei;Ma, Yingfei
通讯作者：
Ma, Yingfei
DOI：
10.1038/nrurol.2015.292
发表时间：
2016-02
期刊：
Nature reviews. Urology
影响因子：
--
作者：
Alfano M;Canducci F;Nebuloni M;Clementi M;Montorsi F;Salonia A
通讯作者：
Salonia A
影响因子：
48
作者：
Edgar, Robert C.
通讯作者：
Edgar, Robert C.
影响因子：
5.7
作者：
Karstens L;Asquith M;Davin S;Stauffer P;Fair D;Gregory WT;Rosenbaum JT;McWeeney SK;Nardos R
通讯作者：
Nardos R