Efficient production of 2,3-butanediol from cheese whey powder (CWP) solution by Klebsiella pneumoniae through integrating pulsed fed-batch fermentation with a two-stage pH control strategy
Efficient production of 2,3-butanediol from cheese whey powder (CWP) solution by Klebsiella pneumoniae through integrating pulsed fed-batch fermentation with a two-stage pH control strategy
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通过将脉冲补料分批发酵与两阶段 pH 控制策略相结合，利用肺炎克雷伯菌从奶酪乳清粉 (CWP) 溶液中高效生产 2,3-丁二醇
DOI：
10.1016/j.fuel.2017.04.138
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发表时间：
2017-09
期刊：
影响因子：
7.4
通讯作者：
Xiao Dongguang
中科院分区：
文献类型：
--
作者：
Guo Xuewu;Wang Yazhou;Guo Jian;Wang Qing;Zhang Yu;Chen Yefu;Zhang Cuiying;Xiao Dongguang
本研究以乳清粉(CWP)为原料，对几株产2，3-丁二醇(2，3-BD)菌株进行了研究。在乳糖和两株肺炎克雷伯菌CICC 10781和K中对这些菌株进行了产2，3-BD能力的测定。选择催产素CICC 21518例。对发酵性能的综合评价表明，中金公司10781比中金公司21518更适合水煤浆生产2，3-BD。采用响应曲面法对培养基组分进行了优化。Na3C6H5O7.2H2O、CH3COONa和尿素是影响2，3-BD合成的关键因素，其最佳浓度分别为3.65、5.45和0.54g/L。在此基础上，提出了脉冲补料分批发酵的两段pH控制策略，即在发酵前8h内保持pH为6.5，然后调至6.0直至发酵结束。采用该工艺，2，3-BD的最大产量可达57.63 mg/L，为水煤浆工业化生产2，3-BD提供了新的指导。
In this study, several strains were investigated to evaluate their ability to produce 2,3-butanediol (2,3-BD) from cheese whey powder (CWP). The capacity of these strains for 2,3-BD production was assessed in lactose medium and two strainsKlebsiella pneumoniaeCICC 10781 andK. oxytocaCICC 21518 were selected. Fully assessing the fermentation performances indicate that CICC 10781 was more suitable for 2,3-BD production from CWP than CICC 21518. Response surface methodology was applied to optimize the medium composition. Na3C6H5O7·2H2O, CH3COONa, and urea were the key factors influencing 2,3-BD synthesis, and the optimal concentrations were 3.65, 5.45, and 0.54 g/L, respectively. Two-stage pH control strategy with pulsed fed-batch fermentation was then developed, in which the pH was maintained at 6.5 for the first 8 h and then shifted to 6.0 till the end of fermentation. Using this strategy, the maximal 2,3-BD production reached 57.63 g/L. The results may provide new guidance for industrial-scale production of 2,3-BD from CWP.
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