Mycobacteriophage Fruitloop gp52 inactivates Wag31 (DivIVA) to prevent heterotypic superinfection.
Mycobacteriophage Fruitloop gp52 inactivates Wag31 (DivIVA) to prevent heterotypic superinfection.
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DOI：
10.1111/mmi.13946
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发表时间：
2018-05
影响因子：
3.6
通讯作者：
Hatfull GF
中科院分区：
文献类型：
--
作者：
Ko CC;Hatfull GF
Bacteriophages engage in complex dynamic interactions with their bacterial hosts and with each other. Bacteria have numerous mechanisms to resist phage infection, and phages must co-evolve by overcoming bacterial resistance or by choosing an alternative host. Phages also compete with each other, both during lysogeny by prophage-mediated defense against viral attack, and by superinfection exclusion during lytic replication. Phages are enormously diverse genetically and are replete with small genes of unknown function, many of which are not required for lytic growth, but which may modulate these bacteria-phage and phage-phage dynamics. Using cellular toxicity of phage gene overexpression as an assay, we identified the 93-residue protein gp52 encoded by Cluster F mycobacteriophage Fruitloop. The toxicity of Fruitloop gp52 overexpression results from interaction with and inactivation of Wag31 (DivIVA), an essential Mycobacterium smegmatis protein organizing cell wall biosynthesis at the growing cellular poles. Fruitloop gene 52 is expressed early in lytic growth, and is not required for normal Fruitloop lytic replication, but interferes with Subcluster B2 phages such as Hedgerow and Rosebush. We conclude that Hedgerow and Rosebush are Wag31-dependent phages, and that Fruitloop gp52 confers heterotypic superinfection exclusion by inactivating Wag31. Expression of gene 52 of mycobacteriophage Fruitloop leads to death of Mycobacterium smegmatis. Fruitloop gp52 is a 93-residue protein that interacts with Wag31, a host protein proposed to be an organizer of cell wall biosynthetic machinery at the growing pole of the cell. Wag31 is an essential gene in M. smegmatis and a potential target for anti-tuberculosis drugs. Fruitloop gp52 is expressed in lytic growth and inactivates Wag31, preventing superinfection by Wag31-dependent phages.
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DOI：
10.4161/bact.18778
发表时间：
2012-01-01
期刊：
Bacteriophage
影响因子：
--
作者：
Marinelli LJ;Hatfull GF;Piuri M
通讯作者：
Piuri M
影响因子：
3.6
作者：
Ginda, Katarzyna;Bezulska, Martyna;Jakimowicz, Dagmara
通讯作者：
Jakimowicz, Dagmara
影响因子：
5.6
作者：
ARBER, W;DUSSOIX, D
通讯作者：
DUSSOIX, D
DOI：
10.1073/pnas.0808832106
发表时间：
2009-01-20
影响因子：
11.1
作者：
Fineran, Peter C.;Blower, Tim R.;Salmond, George P. C.
通讯作者：
Salmond, George P. C.
影响因子：
3.7
作者：
Bergua, Maria;Kang, Sung-Hwan;Folimonova, Svetlana Y.
通讯作者：
Folimonova, Svetlana Y.