Biomarker quantification by multiplexed quantum dot technology for predicting lymph node metastasis and prognosis in head and neck cancer.
Biomarker quantification by multiplexed quantum dot technology for predicting lymph node metastasis and prognosis in head and neck cancer.
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DOI：
10.18632/oncotarget.9225
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发表时间：
2016-07-12
期刊：
影响因子：
--
通讯作者：
Chen ZG
中科院分区：
文献类型：
--
作者：
Hu Z;Qian G;Müller S;Xu J;Saba NF;Kim S;Chen Z;Jiang N;Wang D;Zhang H;Lane K;Hoyt C;Shin DM;Chen ZG
预测头颈鳞状细胞癌（HNSCC）的淋巴结转移和预后。通过 QD 技术对年龄、性别和等级进行量化的膜性 E-钙粘蛋白和膜性表皮生长因子受体 (EGFR) 的组合比通过传统免疫组织化学 (IHC) 量化的任何单一生物标志物或两种组合生物标志物具有更大的预测能力。该模型的预测能力在另一个独立样本集中得到了验证；该模型对LNM的预测敏感性为87.5%，特异性高达97.4%，准确度为92.9%。此外，较高的膜 E-钙粘蛋白水平与较好的总生存期和无病生存期（OS、DFS；分别为 P = 0.002、0.033）显着相关，而较低的细胞质波形蛋白和膜 EGFR 水平与较好的 OS 显着相关（分别为 P = 0.016 和 0.021）。组合生物标志物对 OS 和 DFS 的预后价值比任何单一生物标志物都强。使用多重量子点 (QD) 同时标记 E-钙粘蛋白、波形蛋白和 EGFR，并以 β-肌动蛋白作为内部对照。训练集中包括来自 97 名 HNSCC 患者的原始组织样本，其中 49 名患有 LNM，48 名没有 LNM。通过 InForm 软件对膜 E-钙粘蛋白、细胞质波形蛋白和膜 EGFR 的水平进行定量，并与临床特征相关。 EGFR 和 E-cadherin 的多重亚细胞 QD 定量是预测 HNSCC 患者 LNM、DFS 和 OS 的潜在策略。
To predict lymph node metastasis and prognosis in head and neck squamous cell carcinoma (HNSCC). The combination of membranous E-cadherin and membranous epidermal growth factor receptor (EGFR) quantified by QD technology with age, gender, and grade had greater predictive power than any of the single biomarkers or the two combined biomarkers quantified by conventional immunohistochemistry (IHC). The predictive power of this model was validated in another independent sample set; the predictive sensitivity of this model for LNM was 87.5%, with specificity up to 97.4%, and accuracy 92.9%. Furthermore, a higher membranous E-cadherin level was significantly correlated with better overall and disease-free survival (OS, DFS; P = 0.002, 0.033, respectively), while lower cytoplasmic vimentin and membranous EGFR levels were significantly correlated with better OS (P = 0.016 and 0.021, respectively). The combined biomarkers showed a stronger prognostic value for OS and DFS than any of the single biomarkers. Multiplexed quantum dots (QDs) were used to simultaneously label E-cadherin, vimentin, and EGFR with β-actin as an internal control. Primary tissue samples from 97 HNSCC patients, 49 with and 48 without LNM were included in the training set. Levels of membranous E-cadherin, cytoplasmic vimentin, and membranous EGFR were quantified by InForm software and correlated with clinical characteristics. Multiplexed subcellular QD quantification of EGFR and E-cadherin is a potential strategy for the prediction of LNM, DFS, and OS of HNSCC patients.
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影响因子：
8.4
作者：
Xu, Jing;Mueller, Susan;Nannapaneni, Sreenivas;Pan, Lin;Wang, Yuxiang;Peng, Xianghong;Wang, Dongsheng;Tighiouart, Mourad;Chen, Zhengjia;Saba, Nabil F.;Beitler, Jonathan J.;Shin, Dong M.;Chen, Zhuo (Georgia)
通讯作者：
Chen, Zhuo (Georgia)
DOI：
10.1186/bcr2416
发表时间：
2009
期刊：
Breast cancer research : BCR
影响因子：
--
作者：
Tomaskovic-Crook E;Thompson EW;Thiery JP
通讯作者：
Thiery JP
DOI：
10.1002/hed.21697
发表时间：
2012-01-01
影响因子：
2.9
作者：
Joo, Young-Hoon;Jung, Chan-Kwon;Kim, Min-Sik
通讯作者：
Kim, Min-Sik
超声检查在颈部淋巴结转移中的作用在头部和颈部临床N0鳞状细胞癌中的作用。
影响因子：
4.9
作者：
Richards, P S;Peacock, T E
通讯作者：
Peacock, T E
影响因子：
3.4
作者：
Kurtz, KA;Hoffman, HT;Robinson, RA
通讯作者：
Robinson, RA