Isolated Effects of Plasma Freezing versus Thawing on Metabolite Stability.
Isolated Effects of Plasma Freezing versus Thawing on Metabolite Stability.
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DOI：
10.3390/metabo12111098
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发表时间：
2022-11-11
期刊：
影响因子：
4.1
通讯作者：
中科院分区：
文献类型：
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作者：
众所周知，冷冻和解冻血浆样品会扰乱代谢物的稳定性。然而，没有研究系统地测试了不同的冷冻和解冻方法对血浆代谢物水平的影响。本研究的目的是通过比较10个冻融循环中冷冻和解冻条件的基质，分离冷冻和解冻对小鼠血浆代谢物水平的影响。我们测试了用液氮（LN2）在- 80°C或- 20°C下冷冻，在室温水中快速解冻或在冰上缓慢解冻。提取血浆样品，通过液相色谱-质谱（LC-MS）测定87种代谢物的相对丰度。观察到处理组代谢物丰度的变化与样品冻结或解冻所需的时间有关。因此，用LN2速冻和用水速融导致代谢物水平的变化很小。相反，在- 20°C冷冻的样品显示出代谢物水平的最大变化，可能是因为冷冻需要大约4小时，而在LN2中瞬间冷冻。总的来说，我们的研究结果表明，血浆样品经过长达10个循环的LN2快速冷冻和室温水速融，表现出显著的代谢组稳定性。
Freezing and thawing plasma samples is known to perturb metabolite stability. However, no study has systematically tested how different freezing and thawing methods affect plasma metabolite levels. The objective of this study was to isolate the effects of freezing from thawing on mouse plasma metabolite levels, by comparing a matrix of freezing and thawing conditions through 10 freeze–thaw cycles. We tested freezing with liquid nitrogen (LN2), at −80 °C, or at −20 °C, and thawing quickly in room temperature water or slowly on ice. Plasma samples were extracted and the relative abundance of 87 metabolites was obtained via liquid chromatography–mass spectrometry (LC–MS). Observed changes in metabolite abundance by treatment group correlated with the amount of time it took for samples to freeze or thaw. Thus, snap-freezing with LN2 and quick-thawing with water led to minimal changes in metabolite levels. Conversely, samples frozen at −20 °C exhibited the most changes in metabolite levels, likely because freezing required about 4 h, versus freezing instantaneously in LN2. Overall, our results show that plasma samples subjected to up to 10 cycles of LN2 snap-freezing with room temperature water quick-thawing exhibit remarkable metabolomic stability.
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存储时间和冻融周期对血清样品稳定性的影响。
影响因子：
3.3
作者：
Cuhadar S;Koseoglu M;Atay A;Dirican A
通讯作者：
Dirican A
影响因子：
3.7
作者：
Breier M;Wahl S;Prehn C;Fugmann M;Ferrari U;Weise M;Banning F;Seissler J;Grallert H;Adamski J;Lechner A
通讯作者：
Lechner A
DOI：
10.1016/j.btre.2015.03.004
发表时间：
2015-06
期刊：
Biotechnology reports (Amsterdam, Netherlands)
影响因子：
--
作者：
Roessl U;Leitgeb S;Nidetzky B
通讯作者：
Nidetzky B
影响因子：
14.9
作者：
Metsalu T;Vilo J
通讯作者：
Vilo J
影响因子：
3.7
作者：
Anton G;Wilson R;Yu ZH;Prehn C;Zukunft S;Adamski J;Heier M;Meisinger C;Römisch-Margl W;Wang-Sattler R;Hveem K;Wolfenbuttel B;Peters A;Kastenmüller G;Waldenberger M
通讯作者：
Waldenberger M