Targeted metabolomics identifies reliable and stable metabolites in human serum and plasma samples.
Targeted metabolomics identifies reliable and stable metabolites in human serum and plasma samples.
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DOI：
10.1371/journal.pone.0089728
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Lechner A
中科院分区：
文献类型：
--
作者：
Breier M;Wahl S;Prehn C;Fugmann M;Ferrari U;Weise M;Banning F;Seissler J;Grallert H;Adamski J;Lechner A
需要关于个体中代谢物水平随时间变化的信息来估计代谢物测量的再现性。在干预研究中，适当判断任何类型的干预引起的变化至关重要。分析前阶段（采集、运输和样本处理）是多中心研究中数据质量的一个特别重要的组成部分。采用靶向LC-MS方法，分析了22例健康受试者空腹血清和血浆样本中代谢产物的可靠性（人内和人间方差、组内相关系数）和稳定性（不同温度下的运输模拟、凝胶屏障采集管的使用、冻融循环）。代谢物测量的可靠性更高，在血清中相比，血浆样本，是最饱和的短链和中链酰基肉毒碱，氨基酸，生物胺，甘油磷脂，鞘脂和己糖。大多数代谢产物在冷包上和室温下在非离心管中可稳定储存24 h。血浆和血清代谢物稳定性显示出良好的一致性。血清代谢物浓度大多不受试管类型和1个或2个冻融循环的影响。假设单个时间点测量足以对大多数代谢物进行靶向代谢组学分析。对于运输，理想情况下，样品应在采集后立即分离并冷冻，因为一些氨基酸和生物胺在冷藏包中3小时内会变得不稳定。血清凝胶屏障管可安全用于该过程，因为它们对大多数代谢物的浓度没有影响。对于大多数代谢物而言，将未离心的样品放在冷包上运输是一种具有成本效益的替代方法。
Information regarding the variability of metabolite levels over time in an individual is required to estimate the reproducibility of metabolite measurements. In intervention studies, it is critical to appropriately judge changes that are elicited by any kind of intervention. The pre-analytic phase (collection, transport and sample processing) is a particularly important component of data quality in multi-center studies. Reliability of metabolites (within-and between-person variance, intraclass correlation coefficient) and stability (shipment simulation at different temperatures, use of gel-barrier collection tubes, freeze-thaw cycles) were analyzed in fasting serum and plasma samples of 22 healthy human subjects using a targeted LC-MS approach. Reliability of metabolite measurements was higher in serum compared to plasma samples and was good in most saturated short-and medium-chain acylcarnitines, amino acids, biogenic amines, glycerophospholipids, sphingolipids and hexose. The majority of metabolites were stable for 24 h on cool packs and at room temperature in non-centrifuged tubes. Plasma and serum metabolite stability showed good coherence. Serum metabolite concentrations were mostly unaffected by tube type and one or two freeze-thaw cycles. A single time point measurement is assumed to be sufficient for a targeted metabolomics analysis of most metabolites. For shipment, samples should ideally be separated and frozen immediately after collection, as some amino acids and biogenic amines become unstable within 3 h on cool packs. Serum gel-barrier tubes can be used safely for this process as they have no effect on concentration in most metabolites. Shipment of non-centrifuged samples on cool packs is a cost-efficient alternative for most metabolites.
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存储时间和冻融周期对血清样品稳定性的影响。
影响因子：
3.3
作者：
Cuhadar S;Koseoglu M;Atay A;Dirican A
通讯作者：
Dirican A
影响因子：
7.7
作者：
Clark, S;Youngman, LD;Collins, R
通讯作者：
Collins, R
影响因子：
3.6
作者：
Menni, Cristina;Zhai, Guangju;MacGregor, Alexander;Prehn, Cornelia;Roemisch-Margl, Werner;Suhre, Karsten;Adamski, Jerzy;Cassidy, Aedin;Illig, Thomas;Spector, Tim D.;Valdes, Ana M.
通讯作者：
Valdes, Ana M.
影响因子：
4.4
作者：
Rosenling, Therese;Slim, Christiaan L.;Bischoff, Rainer
通讯作者：
Bischoff, Rainer
影响因子：
7.4
作者：
Yang, Wei;Chen, Yanhua;Abliz, Zeper
通讯作者：
Abliz, Zeper