The effect of storage time and freeze-thaw cycles on the stability of serum samples.
The effect of storage time and freeze-thaw cycles on the stability of serum samples.
复制标题
存储时间和冻融周期对血清样品稳定性的影响。
DOI：
10.11613/bm.2013.009
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发表时间：
2013
期刊：
影响因子：
3.3
通讯作者：
Dirican A
中科院分区：
文献类型：
--
作者：
Cuhadar S;Koseoglu M;Atay A;Dirican A
对于多中心参考区间研究或流行病学研究，中心实验室长期储存血清标本的最佳方法仍有待确定。我们旨在检查化学分析物在多次冻融和长期储存后的分析稳定性。从15名患者中获得血清样品。在基线测量之后，将每个受试者的血清等分并储存在-20 °C下用于两个实验。将一组血清冷冻保存长达1、2和3个月，然后分析稳定性。另一个实验包括1 - 10次冻融循环。在每个时间点共测定了17种化学分析物。将结果与新鲜样品的初步分析结果进行了比较。根据预期偏倚，在统计学和临床上评价了中位或平均较基线（T0）浓度的变化。在研究的分析物中，天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、肌酸激酶（CK）、γ-谷氨酰转移酶（GGT）、直接胆红素、葡萄糖、肌酐、胆固醇、甘油三酯、高密度脂蛋白（HDL）在所有条件下均稳定。血尿素氮（BUN）、尿酸、总蛋白、白蛋白、总胆红素、钙、乳酸脱氢酶（LD）变化显著（P < 0.005）。因此，考虑到不稳定分析物的变异性，常见的临床化学分析物在血清中于−20 °C储存3个月或长达10次冻融循环后显示出足够的稳定性。尽管如此，这种分析只能在特殊情况下进行，在规划研究时应考虑到这一点。
Optimal storage of serum specimens in central laboratories for a long period for multicenter reference interval studies, or epidemiologic studies remains to be determined. We aimed to examine the analytical stability of chemistry analytes following numerous freeze-thaw and long term storage. Serum samples were obtained from 15 patients. Following baseline measurement, sera of each subject were aliquoted and stored at −20 °C for two experiments. A group of sera were kept frozen for up to 1, 2 and 3 months and then analyzed for stability. The other experiment consisted of one to ten times of freeze and thaw cycles. Total of 17 chemistry analytes were assayed at each time point. The results were compared with those obtained from the initial analysis of fresh samples. Median or mean changes from baseline (T0) concentrations were evaluated both statistically and clinically according to the desirable bias. Of the analytes studied, aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatine kinase (CK), gamma-glutamyl transferase (GGT), direct bilirubin, glucose, creatinine, cholesterol, triglycerides, high density lipoprotein (HDL) were stable in all conditions. Blood urea nitrogen (BUN), uric acid, total protein, albumin, total bilirubin, calcium, lactate dehydrogenase (LD) were changed significantly (P < 0.005). As a result, common clinical chemistry analytes, with considering the variability of unstable analytes, showed adequote stability after 3 months of storage in sera at −20 °C, or up to ten times of freeze-thaw cycle. All the same, such analysis can only be performed for exceptional cases, and this should be taken into account while planning studies.
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影响因子：
5
作者：
Amin, Sanjiv B.;Ahlfors, Charles
通讯作者：
Ahlfors, Charles
DOI：
10.1080/00365510701297480
发表时间：
2007-01-01
影响因子：
2.1
作者：
Gao, Y. -C.;Yuan, Z. -B.;Lu, H. -K.
通讯作者：
Lu, H. -K.
影响因子：
4.5
作者：
Beekhof PK;Gorshunska M;Jansen EH
通讯作者：
Jansen EH
影响因子：
7.7
作者：
Jackson, Chris;Best, Nicky;Elliott, Paul
通讯作者：
Elliott, Paul
影响因子：
3.8
作者：
Cao, EH;Chen, YH;Foster, PR
通讯作者：
Foster, PR